致仔猪痢疾沙门氏菌特异性噬菌体的分离与纯化  

Isolation and purification of bacteriophage against Salmonella associated piglets dysentery

作　　者：席利萌[1] 李陇平[1] 徐坤[1] 张智英[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2012年第5期1-4,10,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省"13115"科技创新重大专项(2009ZDKG-18)

摘　　要：【目的】筛选仔猪痢疾病原菌沙门氏菌特异性噬菌体,为噬菌体制剂的研制和仔猪痢疾的生物防治提供参考。【方法】从患痢疾仔猪粪便中分离致病菌沙门氏菌,鉴定后以其为宿主菌,从生活污水中筛选、纯化出特异性噬菌体,并以金黄色葡萄球菌和大肠杆菌为对照,检测该噬菌体的特异性。【结果】分离到了致病菌沙门氏菌,并得到了纯化的沙门氏菌噬菌体;经检测,该噬菌体只能裂解沙门氏菌,而对金黄色葡萄球菌和大肠杆菌没有作用。【结论】得到了纯化的可裂解沙门氏菌的特异性噬菌体。【Objective】 The study was to screen bacteriophage against Salmonella isolated in domestic sewage,and to provide certain reference for the development of diarrhea and disease prevention in piglets dysentery.【Method】 Salmonella was isolated and identified as host bacteria from the sicken piglet＇s feces,the specific bacteriophage screened from sewage through it;After mixing the isolated phage with Staphylococcus aureus（Sau） and Escherichia coli（Eco）,the specificity of the phage was validated.【Result】 The Salmonella were identified,and the specific phage isolated and purified can not kill Sau and Eco.【Conclusion】 We got the specific bacteriophage which can only kill the Salmonella,and the bacteriophage is specific.

关 键 词：仔猪痢疾 沙门氏菌 噬菌体 

分 类 号：S858.286.4[农业科学—临床兽医学]