不同发育阶段Fmr1基因敲除小鼠睾丸组织iNOS的变化  

Expression of iNOS in the testis of Fmr1 knockout mice in different developmental stages

作　　者：杨乙 刘绪红 林波 谭旭东 陈盛强[3] 孙卫文[3] 黄越玲[3] 戴丽军[3] 

机构地区：[1]深圳龙岗妇幼保健院生殖健康科,广东深圳518172 [2]深圳龙岗平湖人民医院泌尿外科,广东深圳518111 [3]广州医学院第二附属医院神经科学研究所,广东广州510260

出　　处：《中华男科学杂志》2012年第6期487-492,共6页National Journal of Andrology

基　　金：广东省科技计划项目(2008B030301371);深圳市龙岗区科技计划项目(YL-2009021)~~

摘　　要：目的:对不同周龄的Fmr1(fragile X mental retardation 1)基因敲除和野生型雄性小鼠睾丸组织诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)的表达进行分析比较,探讨Fmr1基因敲除小鼠睾丸组织中iNOS表达的差异,为脆性X综合征的研究提供背景资料。方法:不同周龄(4、6、8、10周)的Fmr1基因敲除型和野生型雄性小鼠各6只,先采用PCR技术对基因敲除和野生型小鼠进行基因型鉴定,之后所有小鼠麻醉取睾丸组织,采用石蜡包埋切片免疫组化染色技术对基因敲除和野生型小鼠睾丸iNOS的表达进行检测并作对比分析。结果:iNOS在4周野生型小鼠睾丸间质细胞呈弱阳性表达,在6周小鼠呈阳性表达,在8周和10周小鼠呈强阳性表达,且基因敲除小鼠睾丸的iNOS表达均弱于野生型小鼠。结论:Fmr1基因敲除小鼠在缺失FMR1蛋白(FMRP)后的睾丸组织中iNOS的表达降低。Objective： To analyze the expression of inducible nitric oxide synthase （iNOS） in the testis tissues of Frnrl （fragile X mental retardation 1 ） knockout and wild-type male mice in different developmental stages, and provide background information for researches on fragile X syndrome. Methods ： This study included 4, 6, 8 and 10 weeks old Fmrl knockout and wild-type male mice, 6 in each age group. We identified the genotype of the mice by PCR, and detected and compared the expression of iNOS in the testis tissues of the Fmrl knockout and wild-type mice by immunohistochemistry. Results ： The iNOS expression was weakly positive in the Leydig cells of the 4-week-old mice, moderately positive in the 6-week-old ones, and strongly positive in 8- and 10-week-old ones, sig- nificantly weaker in the Fmrl knockout than in the wild-type ones. Conclusion ： The expression of iNOS significantly decreases in the testis of Fmrl knockout mice, suggesting that iNOS may be involved in the pathogenesis of fragile X syndrome.

关 键 词：脆性X综合征 小鼠 FMR1 睾丸 一氧化氮 诱导型一氧化氮合酶 

分 类 号：R749.94[医药卫生—神经病学与精神病学]