β-葡萄糖苷酶高产菌株的筛选及其性质研究  

Screening of high production strain of β-glucosidase and its enzyme characteristics

作　　者：岳冬冬[1] 刘新育[1] 徐新慧[1] 王明道[1] 王彩澜[1] 陈红歌[1] 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002

出　　处：《河南农业大学学报》2012年第2期173-176,181,共5页Journal of Henan Agricultural University

基　　金：河南省科技攻关项目(2010A180006)

摘　　要：从腐败的纤维质中分离筛选得到10株β-葡萄糖苷酶产生菌,与实验室保存的9株菌株一起做产酶发酵,其中B2酶活性最高,为24.4 U·mL﹣1,经菌种鉴定为黑曲霉,其β-葡萄糖苷酶最适合的pH值为4.5,最适温度为65℃,在pH值3.0～10.0较稳定;温度稳定性较好,在65℃保温120 min后仍有61.6%的相对活性;终浓度为5 mmol·L﹣1的Fe2+和Mn2+对酶活性有较强的促进作用.该酶较好的热稳定性和pH值稳定性使其显示出较好的应用潜力.Ten β-glucosidase producing strains were isolated from the cellulosic samples.The highest production strain B2 was finally obtained by screening these 10 strains along with the other 9 strains identified previously as β-glucosidase producers.The B2,identified as Aspergillus niger,showed the yield of β-glucosidase of 24.4 U·mL﹣1 under certain conditions.The optimal pH-value of β-glucosidase was 4.5 and the optimal temperature was 65 ℃.The enzyme was stable in the wide range of pH-value 3.0~10.0.It could retain 61.6% relative activity after incubating 120 min at 65℃,indicating it had a good temperature tolerance.The enzyme was activated significantly by 5 mmol·L﹣1 Fe2＋ and 5 mmol·L﹣1 Mn2＋.The good pH-value stability and thermal stability of this β-glucosidase showed its potential applications in industry.

关 键 词：Β-葡萄糖苷酶 黑曲霉 筛选 

分 类 号：TS201.3[轻工技术与工程—食品科学]