紫甘蓝花色苷的分离纯化  

Purif ication of purple cabbage anthocyains

作　　者：朱振宝[1] 吴园芳[1] 易建华[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,西安710021

出　　处：《食品科技》2012年第6期239-243,共5页Food Science and Technology

基　　金：陕西省教育厅自然科学专项(2010JK454);陕西科技大学自选项目(ZX09-22)

摘　　要：初步研究了紫甘蓝花色苷在SephadexLH-20凝胶层析柱上最佳的分离条件,以及在最佳条件下收集合并各组分,并进行紫外可见全扫描、薄层色谱实验。结果表明:当以30%乙醇为洗脱液,流速为1mL/min时,紫甘蓝花色苷的分离效果最好,共收集合并4个组分。紫外可见全扫描确定Ⅰ组分为非花色苷,Ⅱ、Ⅲ、Ⅳ组分为花色苷,薄层色谱实验表明Ⅱ、Ⅲ组分分别分离出2组花色苷,Ⅳ组分分离出3组花色苷。This work was conducted to research the condition of purification, UV-Visspectrum and thin layer chromatography, which the purple cabbage anthocyains was purifited by sephadex LH-20. It was shown that optimum purification condition was elute with 30% ethanol with a flow rate of 1 mL/min, and collected four pigments, Ⅰ pigment not belonged to anthocyains, and Ⅱ, Ⅲ, IV pigment belonged to anthocyains by UV-Visspectrum; Ⅱ,Ⅲ pigment sepatated 2 anthocyains respectively, and IV pigment sepatated 3 anthocyains by thin layer chromatography.

关 键 词：紫甘蓝 花色苷 SephadexLH-20 紫外可见全扫描 薄层色谱 

分 类 号：R284.2[医药卫生—中药学]