高效液相色谱法测定酵母粉中硫胺素  

Determination of Thia mine Content in Yeast Powder by High Performance Liquid Chromatography

作　　者：纪龙翔[1] 黄倩[1] 张建璀[1] 赵章锁[1] 王玉娟[1] 刘国生[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《安徽农业科学》2012年第17期9464-9466,共3页Journal of Anhui Agricultural Sciences

基　　金：河南省教育厅自然科学基金项目(2011B180032)

摘　　要：[目的]实现企业在肌苷发酵生产中对酵母粉原料质量的评价,为肌苷发酵的控制奠定重要的方法学基础。[方法]将酵母粉经过高温高压并用盐酸提取,通过试验确定高效液相色谱法分离条件并测定硫胺素含量。[结果]采用Hypersil BDS C18色谱柱,紫外检测波长为254 nm;以0.02 mol/L pH 3的磷酸盐缓冲液为流动相,流速1.0 ml/min对提取液进行色谱测定,可以将硫胺素与其他成分有效分离。用0.1 mol/L HCl悬浮后在超声清洗器中振荡10 min混合均匀,然后于121℃高温高压下30 min离心可将酵母粉中的硫胺素充分释放出来。[结论]采用高效液相色谱法测定酵母粉中硫胺素含量,该方法快速、准确,测定结果可做为评价酵母粉质量的指标,对肌苷发酵有重要指导意义。[ Objective ] To evaluate the quality of yeast powder for the industrialized production of inosine fermentation, so as to lay methodologi- cal basis for the control of inosine fermentation. [ Method ] After high temperature and high pressure treatment, the yeast powder was extracted with HC1, the HPLC conditions were determined through experiment and the content of thiamine was measured. [ Result ] The extraction liquid was chromatographically determined with Hypersil BDS C18 column at the UV detection wavelength of 254 nm and with 0.02 mol/L and pH 3 phosphate buffer as the mobile phases at a flow rate of 1.0 ml/min. The thiamine can be effectively separated from the other ingredients under the chromatographic conditions. The yeast extract which was suspended with 0.1 mol/L HC1 was shocked by the ultrasonic cleaner for 10 min to mix well, and then centrifuged for 30 min at 121 ~C, thus the thiamine was fully extracted out, [ Conclusion] The HPLC method is rapid and accu- rate, its determination results can be used to evaluate the quality of yeast powder and has great significance to inosine fermentation.

关 键 词：高效液相色谱 硫胺素 酵母粉 肌苷发酵 

分 类 号：S121[农业科学—农业基础科学]