高效离子色谱法测定乳及乳制品中低聚果糖含量  

Determination of Fructooligosaccharides in Milk and Milk Products by High Performance Ion Exchange Chromatography

作　　者：刘玉峰[1] 李东[1] 唐华澄[1] 张绩觅[1] 

机构地区：[1]北京市营养源研究所分析检测中心,北京100069

出　　处：《乳业科学与技术》2012年第1期40-42,共3页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

摘　　要：建立离子色谱法定量测定乳及乳制品中低聚果糖的方法,应用CarboPacTMPA1(4mm×250mm)阴离子交换柱、氢氧化钠和醋酸钠淋洗液梯度淋洗、脉冲安培检测器检测。6次测定液态奶中低聚果糖的含量,相对标准偏差小于9%,3种糖的加标回收率在94.8%～103.6%之间。本方法简单快捷,适用于乳及乳制品中低聚果糖含量的检测。An analytical method was established to determine fructooligosaccharides （FOS） in milk and milk products by high performance ion exchange chromatography method with pulsed amperometric detection. The column was a CarboPac PAl （4 mm x 250 mm） anion exchange column. Fructooligosaccharides were separated under gradient elution mode. The mobile phase A was 150 mmol/L NaOH and the mobile phased B was composed of 150 mmol/L NaOH and 100 mmol/L sodium acetate. The relative standard derivations for 6 replicate determinations of four fructooligosaccharides in liquid milk were less than 9%. The average spike recovery rates for three fructooligosaccharides in liquid milk varied between 94.8% and 103.6%. The method was simple, fast and suitable for determining FOS in milk and milk products.

关 键 词：高效离子色谱法 脉冲安培检测 低聚果糖 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.75[轻工技术与工程—食品科学与工程]