黄曲霉菌株的分离、鉴定及产毒能力分析  

Isolation and Identification of Aspergillus Flavus Strains and Analysis of Toxin-Producing Ability

作　　者：杨生瑞[1,2] 屈凌波[1] 孙长坡[2] 沈晗[2] 沈瑾[3] 伍松陵[2] 

机构地区：[1]河南工业大学生物工程学院,郑州450001 [2]国家粮食局科学研究院,北京100037 [3]农业部规划设计研究院农副产品加工研究所,北京100126

出　　处：《中国粮油学报》2012年第6期110-114,123,共6页Journal of the Chinese Cereals and Oils Association

基　　金：国家科技支撑计划(2009BADA0B05);公益性行业(农业)科研专项(201003077)

摘　　要：对几株从发霉粮食中分离出的黄曲霉菌菌株进行形态学和分子生物学鉴定,并进行发酵培养和产毒能力的HPLC测定。结果表明:试验分离菌株均为黄曲霉菌株且含有黄曲霉毒素产生的关键基因aflR;黄曲霉菌株之间产毒能力差异巨大:黄曲霉菌株3.4408产毒量最高,黄曲霉菌株HDWS产毒量最低,黄曲霉菌株3.2572甚至不产生黄曲霉毒素;产生黄曲霉毒素菌株中部分黄曲霉菌株产生4种黄曲霉毒素AFB1、AFB2、AFG1、AFG2,黄曲霉菌株HDWH只产生黄曲霉毒素AFB1、AFB2。Several Aspergillus flavus strains were isolated from moldy grain, then were identified by spore mor- phological and Molecular Biological Identification. The toxin - producing ability of strains were detected and analyzed by HPLC after fermentation. The results showed that experimental isolated strain were Aspergillus flavus and contains toxigenic key gene of aft; the toxin - producing ability was very different between these Aspergillus flavus strains. As- pergillus flavus 3. 4408 produce the hightest aflatoxin while Aspergillus flavus HDWS only produces a little. Even more, Aspergillus flavus 3. 2572 could not produce aflatoxin under the same condition; Among aflatoxin -producing strains, some of the strains produce aflatoxin AFB1, AFB2, AFG1, AFG2 ,Aspergillus flavus HDWH only produces aflatoxin AFB1, AFB2.

关 键 词：黄曲霉菌 黄曲霉毒素 aflR基因 高效液相色谱 

分 类 号：Q93-31[生物学—微生物学]