低氧联合NaCl胁迫下蚕豆发芽富集γ-氨基丁酸培养条件优化  

The Optimization of Culture Condition on γ-aminobutyric Acid Accumulation in Fava Bean (Vicia faba L.) Under Hypoxia Combined with NaCl

作　　者：杨润强[1] 陈惠[1] 顾振新[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品与发酵工业》2012年第5期77-80,共4页Food and Fermentation Industries

基　　金：国家自然科学基金(31071581);中央高校科研业务费专项基金(KYZ200917);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：以蚕豆(启豆2号)为原料,研究了低氧联合NaCl胁迫下培养条件对γ-氨基丁酸(GABA)富集的影响。结果显示:非胁迫培养时间、培养pH和胁迫培养时间显著影响发芽蚕豆GABA积累。蚕豆发芽富集GABA最佳培养条件是非胁迫培养1.5 d、培养液pH 3.5和低氧联合NaCl胁迫4 d,在此条件下其GABA含量可达1.06mg/g DW,为原料蚕豆的7.57倍。The culture condition of γ-aminobutyric acid accumulation in germinating fava bean （Qi Bean 2 ） seeds under hypoxia combined with NaC1 was investigated. The results showed that non-stress culture time, pH and stress time impacted the accumulation of GABA significantly. The optimum condition was culturing for 1.5 days, pH 3.5 and stress for 4 days. The GABA content was 1.06 mg/g DW and was 7.57-fold of that in un-germinated lava bean.

关 键 词：蚕豆 发芽 Γ-氨基丁酸 富集 低氧胁迫 NaC1胁迫 

分 类 号：TQ922[轻工技术与工程—发酵工程]