3种菌株产木聚糖酶同工酶及其酶解产物的比较  

Comparative Study on Xylanase Isozymes and Their Hydrolysates from Three Different Strains

作　　者：王贝[1] 王俊丽[1] 闫潇[1] 张建新[1] 侯彩霞[1] 聂国兴[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《西北农业学报》2012年第4期159-163,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：河南省高校科技创新杰出人才项目(2010HASTIT020);河南省科技攻关重点项目(112102210106)

摘　　要：探讨3种菌株所产木聚糖酶组分及其酶解产物的差异,为酶制剂和功能低聚木糖的研发和应用提供理论依据。采用SDS-PAGE电泳,研究黑曲霉C71、康宁木霉M46及XYNB重组工程菌G81所产的木聚糖酶同工酶。结果表明,C71、M46及G81分别产3种、4种和2种木聚糖酶同工酶;以桦木木聚糖为底物,在酶添加量为70U/g、底物20g/L、反应时间10h条件下进行酶解,采用TLC及HPLC分析酶解产物。TLC结果表明,C71和M46酶解液中含有木糖、木二糖和木三糖;G81酶解液中含有木二糖、木三糖和木四糖。HPLC结果表明,C71酶解液中木糖、木二糖和木三糖的质量浓度分别为2.4、1.3和1.7g/L;M46酶解液中木糖、木二糖和木三糖的质量浓度分别为1.5、1.8和2.1g/L;G81酶解液中木二糖、木三糖和木四糖的质量浓度分别为2.6、2.7和1.4g/L。不同菌株所产木聚糖酶组分及其酶解产物存在差异,低聚木糖的研发应根据酶学特性,选择不同的单一或复合酶制剂。Xylanases were produced by Aspergillus niger C71,Trichoderma koningii M46 and recombinant XYNB C81.These xylanase isozymes were analyzed with SDS-PAGE electrophoresis.The hydrolyzing conditions of xylanases were as follow： substrate is birchwood,dosage of enzyme is 70 U/g,substrate concentration is 20 g/L,the hydrolysis time is 10 h.The hydrolysates of xylanases from three different strains were detected by using TLC and HPLC.The results showed that the components of xylanase were N-1,N-2,N-3（produced by Aspergillus niger G71）;Y-1,Y-2,Y-3,Y-4（produced by Trichoderma koningii M46） and Z-1,Z-2（produced by the recombinant XYNB G81）.TLC and HPLC analysis indicated that the hydrolysates of xylanases produced by A.niger C71 and Trichoderma reesi M46 contained xylose（2.4 g/L,1.5 g/L,respectively）,xylobiose（1.3 g/L,1.8 g/L,respectively） and xylotriose（1.7 g/L,2.1 g/L,respectively）.The hydrolysates of xylanase produced by XYNB G81 contained xylobiose（2.6 g/L）,xylotriose（2.7 g/L） and xylotetraose（1.4 g/L）.The experimental results provided theoretical basis for the development of XOS.

关 键 词：木聚糖酶 同工酶 酶解产物 TLC HPLC 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]