不同生境产脂肪酶菌株的筛选及多样性  

Isolation and Diversity Analysis of Lipase-producing Strains in Different Habitats

作　　者：徐辉[1] 林家富[1] 费忠安[1] 费保进[1] 余英鹏[1] 乔代蓉[1] 曹毅[1] 

机构地区：[1]四川大学生命科学学院微生物与代谢工程四川省重点实验室,成都610065

出　　处：《应用与环境生物学报》2012年第3期455-459,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家基础性工作专项(No.2008FY110400-4-1);四川省科技支撑计划项目(Nos.2010GZ0290;11DXYD167JH)资助~~

摘　　要：为发掘不同特性脂肪酶微生物资源,采用纯培养方法从青海高寒草地、雅安山区、成都平原分离得到产脂肪酶菌株54株.通过对菌株的DNA进行ERIC-PCR指纹图谱分析,按聚类树划分为5个操作分类单元.16S rDNA序列测定和聚类分析显示,分离菌株分布于芽孢杆菌属(Bacillus)、克雷伯氏菌属(Klebsiella)、假单胞菌属(Pseudomonas)、葡萄球菌属(Staphylococcus)、肠杆菌属(Enterobacter).多样性分析表明,与青海高寒草地、雅安山区相比,成都平原产脂肪酶菌株遗传多样性更为丰富.在54株菌中都出现了300 bp和1 300 bp两个特异且稳定出现的条带,这两个特异条带可能是产脂肪酶菌株的SCAR标记条带.In order to explore lipase-producing microbes with different characteristics,54 lipase-producing strains were screened out using pure culture method from 108 samples from the Qinghai alpine meadow – Ya’an Mountainous Regions Chengdu Plain,and only one strain is alkaline lipase-producing strain.All the ERIC-PCR fingerprints were obtained,and the tree diagram was divided into five operation units according to the fingerprints.The results showed that there existed more rich genetic diversity in all the isolates from Chengdu Plain than from Tibetan Plateau and Ya’an Mountainous Regions.The isolated strains distributed in Bacillus,Klebsiella,Pseudomonas,Staphylococcus and Enterobacter.Moreover,the fingerprints displayed different specific bands in different strains,appearing two conservative bands（300 bp and 1 300 bp） which indicated that these two bands might be the SCAR marker of the lipase-producing strains.

关 键 词：产脂肪酶菌株 碱性脂肪酶 ERIC-PCR 青海高寒草地 

分 类 号：TQ925[轻工技术与工程—发酵工程]