广西人肝细胞癌β-catenin基因突变及蛋白表达  

Expression and mutation analysis of β-catenin in patients with hepatocellular carcinoma in Guangxi

作　　者：苗志国[1] 齐鲁楠[2] 朱海[1] 彭涛[3] 黎乐群[2] 

机构地区：[1]广西医科大学研究生学院 [2]广西医科大学附属肿瘤医院肝胆外科,南宁530021 [3]广西医科大学第一附属医院肝胆血管外科

出　　处：《中国癌症防治杂志》2012年第2期119-122,共4页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：国家自然科学基金资助项目(30960021);广西自然科学基金资助项目(桂科基0640101);广西科技攻关资助项目(桂科攻0632007-1E)

摘　　要：目的研究广西地区人肝细胞癌发生、发展过程中β-catenin基因突变和蛋白表达状况。方法采用PCR联合基因直接测序法,检测108例肝细胞癌癌组织中β-catenin基因的突变;同时采用免疫组化方法检测β-catenin蛋白的表达状况。结果 108例HCC癌组织中仅有12例发生β-catenin基因突变,突变率为11.1%。108例HCC癌组织中β-catenin蛋白阳性表达率为70.0%(75/108),其中14.6%(11/75)为细胞核阳性;57.3%(43/75)为细胞质阳性;28.0%(21/75)为细胞膜阳性。β-catenin蛋白在12例有突变的样本中表达阳性率为100%。结论广西地区肝癌中β-catenin基因突变率比较低;β-catenin基因突变可能为导致β-catenin蛋白过表达的因素之一,并参与了肝癌癌变过程。Objective To explore β-catenin gene mutations and protein expression in patients with hepatocellular carcinoma（HCC） in Guangxi. Methods β-catenin gene mutations and protein expression were analyzed in 108 HCC tissues by direct sequencing and immunohistochemistry, respectively. Results Mutations in the β-catenin gene were found in 12 of 108 HCC tissues, giving a mutation rate of 11.1% .The overall rate of positive β-catenin protein expression was 70.0% ,including samples that were nuclear-positive （14.6%）,cytoplasm-positive （57.3%）and membrane-positive （28.0%）.The protein was expressed in 100% of the tissues carrying β- catenin gene mutations. Conclusions HCC patients in Guangxi show a relatively low rate of β-catenin gene mutation.These muta- tions may play an import role in hepatocarcinogenesis by causing over-expression of β-catenin protein.

关 键 词：肝肿瘤 Β-CATENIN 突变 

分 类 号：R735.7[医药卫生—肿瘤]