不对称还原4’-甲基苯乙酮微生物的筛选及反应条件优化  

Screening of strain for asymmetric reduction of 4'-methylacetophenone and optimization of reaction conditions

作　　者：卓梅芳[1] 马丽[1] 刘雄民[1] 张笮晦[1,2] 库咏峰[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004 [2]广西水产研究所,广西南宁530021

出　　处：《食品工业科技》2012年第13期184-186,204,共4页Science and Technology of Food Industry

基　　金：广西科学基金项目(桂科基0832002);广西科学研究与技术开发计划项目(桂科攻1099061-2)

摘　　要：从土壤中筛选得到一株能将4’-甲基苯乙酮高效不对称还原为S-4’-甲基-1-苯乙醇的菌株T19,T19采用16SrDNA进行分子生物学鉴定,被鉴定为卷枝毛霉菌(Mucor circinelloides)。研究表明,在体系pH为6～7,反应温度30℃的条件下,添加50g/L的葡萄糖为辅助底物,底物4’-甲基苯乙酮的加入量为3g/L,反应3d后,产物S-4’-甲基-1-苯乙醇的得率最高可达92.6%,对映体过量值(e.e.)达到100%。A strain T19 was screened from soil for asymmetric reduction of 4＇-methylacetophenone to S-4＇- methyl-phenylethyl alcohol. The strain T19 was identified as Mucor circinelloides according to the 16S rDNA sequences. When pH6N7,30℃and 50g/L glucose was added as the cosubstrate,the addition of 4＇- methylacetophenone was 3g/L. After three days of reaction,the yield of S-4＇-methyl-phenythyl alcohol was reached 92.6% and e.e. was 100%.

关 键 词：4’-甲基苯乙酮 不对称还原 卷枝毛霉 S-4’-甲基-1-苯乙醇 

分 类 号：TS201.2[轻工技术与工程—食品科学]