原子力显微镜在细胞与生物大分子超微结构和力学研究中的应用  

Application of atomic force microscopy in ultrastructure and biomechanics of cells and biomacromolecules

作　　者：朱杰[1,2,3] 郭连红[1,4] 王国栋[1] 欧阳五庆[2] 

机构地区：[1]西北农林科技大学理学院,生物物理研究所,心血管生物力学实验室,杨凌712100 [2]西北农林科技大学动物医学院,杨凌712100 [3]吉林大学超分子结构与材料国家重点实验室,长春130012 [4]中南大学公共卫生学院,长沙410078

出　　处：《医用生物力学》2012年第3期355-360,共6页Journal of Medical Biomechanics

基　　金：中国博士后科学基金(20100481372);中央高校基本科研业务费专项资金项目(QN2011123);超分子结构与材料国家重点实验室开放课题(SKLSSM201127);西北农林科技大学留学归国人员科研启动费(Z111020906)

摘　　要：作为微纳米科学理论与技术迅猛发展的代表,原子力显微镜(atomic force microscopy,AFM)在其25年的发展过程中极大地推进了生物学在微纳米尺度上的拓展,为微纳米生物学的诞生与发展提供了重要技术手段。本文在介绍AFM基本原理和检测模式的基础上,结合作者在该领域的研究成果和工作经验,从生物结构与形态学研究、表面物化性质表征、生物大分子的力学操纵三方面综述了AFM在细胞与生物大分子超微结构与生物力学特性研究中的具体应用,并重点探讨了AFM在细胞与生物大分子科学研究中亟待改进和解决的科学与技术问题,提出了一些探讨性的见解和建议。To be the representative fruition resulted from the rapid development in micro-nano theory and technology, atomic force microscopy （AFM） has greatly promoted the expansion of biological research in micro-nano scale, and facilitated the birth and development of micro-nano biology as an important technique in its 25-year evolutional progress. Based on the fundamental principles and detection modes of AFM, as well as the author＇ s re- search findings and work experience in this field, the paper reviews the application of AFM in the study on ultra- structure and biomechanical properties of cells and biomacromolecules in the aspects of biological structure and morphology, surface physicochemical characterization and mechanical manipulation of biological macromote- cules, and focus on some important scientific and technical problems on AFM in micro-nano biomedical research needed to be improved and solved urgently, with exploratory insights and recommendations for potential users in ultrastructure and biomechanics of cells and biomacromolecules.

关 键 词：原子力显微镜 微纳米生物学 超微结构 物化特性 生物力学 

分 类 号：Q66[生物学—生物物理学]