粘附分子CD44v6表达和p53基因突变与卵巢癌转移关系的研究  

Study on the Relationship between CD44v6,p53 Gene Mutation and Ovarian Carcinoma Metastasis

作　　者：孙宜[1] 沈仲毅[2] 季晓琼[1] 

机构地区：[1]上海第二医科大学附属上海瑞金医院妇产科,200025 [2]上海第二医科大学上海市免疫学研究所

出　　处：《中华妇产科杂志》2000年第4期225-228,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　探索卵巢癌粘附分子CD44的变构体CD44v6表达和p5 3基因突变与卵巢癌转移之间的关系和作用机理。方法　选择正常卵巢 2 0例、良性卵巢肿瘤 2 0例、卵巢癌 45例 (其中 2 0例肿瘤无转移 ,2 5例肿瘤有转移 ) ,应用流式细胞仪测定卵巢癌细胞DNA含量及其在细胞周期各相中的分布 ;应用逆转录 聚合酶链反应 (RT PCR)及特异探针D3 对卵巢癌细胞进行DNA印渍杂交分析 ,并对杂交条带进行辉度扫描 ;应用银染聚合酶链反应 单链构象多态性 (PCR SSCP)技术检测卵巢癌细胞p5 3基因突变 ,并与正常卵巢和良性卵巢肿瘤进行比较。结果　正常卵巢、良性卵巢肿瘤、卵巢癌无转移和有转移者CD44v6表达阳性率分别为 0 %、10 %、75 %、88% ;而p5 3基因突变率分别为 0 %、5 %、40 %、6 0 % ,两者都呈逐渐上升趋势。CD44v6辉度扫描 ,卵巢癌无转移者 (382 0± 2 89)与有转移者(10 132± 15 2 1)比较 ,差异有极显著性 (P <0 .0 1)。卵巢癌CD44v6表达阳性和阴性患者中 ,G2 M期细胞平均值分别为 5 .90 %和 5 .0 6 % (P >0 .0 5 ) ;异倍体细胞分别为 5 7%和 5 0 % (P >0 .0 5 )。而在p5 3基因有突变和无突变患者中 ,G2 M期细胞分别为 11.15 %和 5 .85 % (P <0 .0 5 ) ;异倍体细胞分别为 74%和 36 % (P <0 .0 5 )。Objective To explore the relationship between CD44v6 expression, p53 gene mutation, cell ploidy and ovarian metastasis. Methods Eighty five ovarian specimens (20 normal specimens, 20 benign tumors, 20 non metastatic carcinomas and 25 metastatic carcinomas) were collected. Cell ploidy and the distribution of cell population in each stage of cell cycle were detected by flow cytometry. By using techniques such as reverse transcription polymerase chain reaction, southern blot and image analysis, the quantitative and qualitative expression of CD44v6 were determined; the mutation of p53 gene in the ovarian specimens was also determined by polymerase chain reaction single strand conformation polymorphism with silver staining. Results In the normal ovarian specimens, benign tumors, non metastatic and metastatic carcinomas, the positive rates of CD44v6 expression and p53 gene mutation were 0%, 10%, 75%, 88% and 0%, 5%, 40%, 60%,respectively, both increased gradually. The mean dark density in the non metastatic (3 820±289) and metastatic (10 132±1 521) groups was significantly different ( P <0.01).In the positive and negative CD44v6 ovarian carcinoma groups, the mean carcinoma cell percentage at the G 2M stage was 5.90% and 5.06% ( P >0.05), mean aneuploidy carcinoma cell percentage was 57% and 50% ( P >0.05) respectively. In the mutated and non mutated p53 gene groups, their mean cell population at the G 2M stage was 11.15% and 5.85% ( P <0.05), mean aneuploidy carcinoma cell percentage was 74% and 36% ( P <0.05), respectively. Conclusions The expression of CD44v6 is closely related to carcinoma metastasis. The relationship between CD44v6 expression, p53 gene mutation and carcinoma metastasis can be involved in the different mechanism. As compared to mutated p53 gene, CD44v6 appears to be more suitable as a metastatic marker.

关 键 词：卵巢肿瘤 抗原 CD44 基因 p53 倍性 突变 

分 类 号：R737.31[医药卫生—肿瘤]