HBV和AFB_1在树鼠句肝癌发生中的作用  

The effect of HBV and AFB_1 in tree shrew's hepatocarcinogenensis

作　　者：苏建家[1] 覃柳亮[1] 李瑗[1] 班克臣[1] 杨春[1] 严瑞琪[2] 

机构地区：[1]广西肿瘤防治研究所病理室,广西南宁530021 [2]中山医科大学肿瘤研究所,广东广州510060

出　　处：《中国癌症杂志》2000年第2期155-158,共4页China Oncology

基　　金：国家自然科学基金资助项目! (392 6 0 0 33)

摘　　要：目的 :探讨乙肝病毒 (HBV)和黄曲霉毒素B1(AFB1)在肝癌形成中的作用及机理。方法 :用免疫组化、原位杂文及印迹杂交检测树鼠句肝和肿瘤组织。结果 :两次实验中 ,感染HBV又摄入AFB1的A组树鼠句肝细胞癌(HCC)发生率分别为 5 2 94%和 6 6 7% ;单纯感染HBV的B组分别为 11 1%和 0 ;只摄入AFB1的C组分别为 12 5 %和 30 % ;空白对照组D均无HCC发生。A组HCC的发生率均显著高于各组 (P <0 0 5 )。癌前病变r GT灶的个数及面积也如此。IGF Ⅱ的过表达与HCC发生密切有关 ;HBV和AFB1可协同激活ras基因 ;c myc主要参与HCC的演进 ,CDK4参与了HCC的发生和演进过程。这两种基因的改变与HBV感染有关。结论 :HBV能诱发肝癌前病变和肝癌 ,并与AFB1有协同致肝癌作用。Purpose:To detect the effect and mechanism in hepatocarcinogenesis induced by HBV and aflatoxin B 1 (AFB 1) in tree shrews, two animal experiments had been done. Methods:The animals were divided into four groups: group A was infected with HBV and given AFB1; group B only infected with HBV; group C exposed to AFB 1 alone and Group D used as control. The livers and tumors were detected by immunohistochemistry, in situ hybridization and southern blot. Results:In both of the experiments, the incidence of hepatocellular carcinoma (HCC) in the group A was always higher (52.94% and 66.7%) than that in group B (11.1% and 0) and C (12.5% and 30%). No HCC developed in group D. The number of GGT positive foci, the premalignant alteration, was also significantly higher in group A than that in group B, C and D. The overexpression of IGF Ⅱ was correlated wity the development of HCC. There is a synergistic activation of ras gene by the factors of HBV and AFB 1. The activation of c myc gene took part in the progress of HCC. in tree shrew's hepatocarcinogenesis, demonstrate an etiological relationship between HBV and HCC. CDK 4 genes were related with HBV infection. Conclusions:There is remarkable synergistic effect between HBV and AFB 1. There are many genes involved in tree shrew's hepatocarcinogenesis.

关 键 词：乙型肝炎病毒 黄曲霉毒素B1 肝癌 树QU 

分 类 号：R735.702[医药卫生—肿瘤]