K-ras基因突变在内蒙古地区结直肠癌患者中研究  

K-ras mutation in colorectal cancer in Inner Mongolia

作　　者：韩文生[1,2] 孙素青[1] 徐伟明[1] 娜琴[1] 陈阿梅[1] 

机构地区：[1]内蒙古医学院组织胚胎学教研室,呼和浩特010059 [2]内蒙古医学院第四附属医院内二科

出　　处：《中国优生与遗传杂志》2012年第2期44-46,共3页Chinese Journal of Birth Health & Heredity

基　　金：内蒙古自然科学基金资助项目(2009MS1161)

摘　　要：目的检测内蒙地区结直肠癌K-ras基因突变情况,并结合临床病理资料加以分析。方法提取15例结直肠癌患者结直肠癌手术切除标本组织的DNA,对产物进行基因序列癌组织的DNA聚合酶链反应(PCR)扩增、DNA直接测序分析。结果 K-ras基因突变率为0%,几种分化型的结直肠癌均未发现K-ras基因突变类型,包括12密码子(GGT)、13密码子(GGC)。结论我院结直肠癌患者k-ras基因突变率为0%,转移性结直肠癌患者原发肿瘤与转移灶肿瘤k-ras基因型均相同;结直肠癌患者k-ras基因突变与否与年龄、性别、肿瘤浸润深度、肿瘤组织学类型无关。Objective： To detect K -ras mutation in colorectal cancer in Inner Mongolia and its correlation with parameters of clinical pathology. Methods： DNA from 15 patients with colorectal carcinoma was extracted, K - ras gene was amplified by using polymerase chain reaction （PCR）, and DNA direct sequencing. Mutations in exon 12 were analyzed. Results： 2 kinds of K -ras gene were found, namely, codon 12 （GGT） , codon 13 （GGC）. The frequency of K - ras gene mutation in colorectal carcinoma was 0% in Inner mongolia. Conclusion： The frequency of K -ras gene mutation in coloreetal carcinoma was 0% in Inner mongolia. The frequency of K - ras mutation is no correlated with the age, the position, the invasion depth, the histologic types.

关 键 词：结直肠癌 K-RAS基因 突变 

分 类 号：R735.3[医药卫生—肿瘤]