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作 者:秦宁[1] 徐日福[1] 张树敏[2] 李莫南[1] 包艳波[1] 王志贤[1] 姜建萍[1]
机构地区:[1]吉林农业大学动物科学技术学院,长春130118 [2]吉林省农业科学院畜牧分院,公主岭136100
出 处:《吉林农业大学学报》2012年第3期343-347,共5页Journal of Jilin Agricultural University
基 金:国家转基因生物新品种培育重大专项(2011ZX08006-002;2008ZX08006-002);国家自然科学基金项目(30871780)
摘 要:采用石蜡切片技术和光学显微数字成像方法观察长白山野猪和东北民猪骨骼肌组织的肌纤维形态特点,分别对两品种各30头供试猪40日龄时背最长肌、股二头肌、股四头肌和三角肌组织的肌纤维直径和密度进行显微测定和分析。结果表明:在4种骨骼肌组织中,东北民猪的肌纤维直径均显著大于长白山野猪的肌纤维直径(P<0.05),其肌纤维密度均显著低于长白山野猪的肌纤维密度(P<0.05)。在同品种内,不同性别猪的同一种骨骼肌组织之间肌纤维的直径和密度差异不显著(P>0.05);在不同品种间,同性别猪同一种组织之间肌纤维的直径和密度差异显著(P<0.05);长白山野猪和东北民猪的肌纤维直径和密度间均呈显著负相关。可见,至40日龄时,猪骨骼肌肌纤维直径和密度的生长发育受品种效应的影响较大,而受性别效应的影响差异不显著。In this study, the technology of paraffin section and the digital imaging optical microscopy was conducted to observe the morphological characteristics of skeletal muscle fibers of Changbaishan wild boars (CW) and Northeast Min pigs (NM), the muscle fiber diameters and densities of the longissimus dorsi, the biceps femoris, the quadriceps and deltoid from two breeds of 30 tested pigs at 40-day-age were deter- mined and analyzed respectively. This result reveals that in the four kinds of skeletal muscle tissues, the diameters of muscle fibers in NM were significantly higher than those of CW breed ( P 〈 0.05), the fiber densities of NM were significantly thinner than those of CW ( P 〈 0.05) ; for the same breed, the diame-ter difference and density differences between different gender individuals were not significant ( P 〉 0.05) in the same kind of skeletal tissues, respectively. For the same kind of skeletal tissues from the same gender, both the muscle fiber diameter difference and density differences between different porcine breeds were significantly different( P 〈 0.05), respectively. The muscle fiber diameters and densities of both CW and NM were significantly negatively correlated. Above all, the postnatal growth and development of the diameters and densities of the pigs' skeletal muscle fiber were considerably affected by breed effects, but the gender effect was not significant.
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