Determination of luteolin and acteoside in Siphonostegiae Herba by high-performance liquid chromatography  

高效液相色谱法测定北刘寄奴药材中木犀草素和毛蕊花糖苷的含量(英文)

作　　者：赵明波[1] 魏荷琳[1] 李军[2] 屠鹏飞[1] 

机构地区：[1]北京大学医学部天然药物及仿生药物国家重点实验室,药学院天然药物学系,北京100191 [2]北京中医药大学中药现代研究中心,北京100029

出　　处：《Journal of Chinese Pharmaceutical Sciences》2012年第4期333-337,共5页中国药学（英文版）

基　　金：National Key Technology R&D Program "New Drug Innovation" of China (Grant No. 2009ZX 09308-004,2009ZX09311-004,2012ZX09301002-002)

摘　　要：A sensitive RP-HPLC-DAD method has been developed and validated for the determination of luteolin and acteoside in the herb ofSiphonostegia chinensis Benth. （Siphonostegiae Herba）. Separation was achieved on an Agilent Zorbax SB-Aq C18 column （250 mm×4.6 mm, 5 μm） using a gradient elution with mobile phases of 0.05% phosphoric acid aqueous solution （A） and methanol （B）. The assay was carried out at a flow rate of 1.0 mL/min with detection at 310 nm and 350 nm. Luteolin and acteoside showed good linearity in the ranges of 0.0341-0.8172 mg/mL （r2 = 0.9999） and 0.0708-2.832 mg/mL （r2 = 0.9999） with average recoveries of 102.7% and 98.3%, respectively. The contents of luteolin and acteoside varied greatly in 15 samples from different habitats. This is the first report on the quantitative determination of acteoside in Siphonostegiae Herba.建立了北刘寄奴药材中木犀草素和毛蕊花糖苷的反相高效液相含量测定方法。采用Agilent Zorbax SB-Aq C18 column (250 mm×4.6 mm, 5 μm) 色谱柱; 0.05%磷酸 (A) 和甲醇 (B) 梯度洗脱; 流速1.0 mL/min; 检测波长310 nm和350 nm。木犀草素和毛蕊花糖苷分别在0.0341-0.8172 mg/mL (r2 = 0.9999)和0.0708-2.832 mg/mL (r2 = 0.9999)范围内与峰面积呈良好的线性关系。平均加样回收率分别为102.7%和98.3%。15批北刘寄奴药材中木犀草素和毛蕊花糖苷的含量差异明显。本文首次报道了北刘寄奴药材中毛蕊花糖苷的含量测定方法。

关 键 词：Siphonostegiae Herba Siphonostegia chinensis Benth. LUTEOLIN ACTEOSIDE HPLC-DAD 

分 类 号：R284.1[医药卫生—中药学]