河南贲门癌高发区贲门癌组织浸润淋巴细胞CD4、CD8、γδT和肿瘤细胞P53的表达及意义  

Alterations of CD4,CD8 and γδT infiltrating T lymphocytes and P53 tumor cell in gastric cardia adenocarcinoma(GCA) from high incidence area for GCA in Henan,China

作　　者：江亚南[1] 范宗民[2] 王苒[2] 任景丽[3] 王立东[2] 

机构地区：[1]郑州大学基础医学院病理生理教研室,450000 [2]河南省食管癌重点开放实验室,郑州市450052 [3]郑州大学第二附属医院病理科,450014

出　　处：《实用医学杂志》2012年第13期2193-2195,共3页The Journal of Practical Medicine

基　　金：河南省医学科技攻关重大项目(编号:WKJ2007-2-028)

摘　　要：目的:探讨河南贲门癌高发区贲门癌组织浸润淋巴细胞CD4、CD8、γδT和肿瘤细胞P53的表达变化特征、相关性及贲门癌组织浸润淋巴细胞CD4、CD8和γδT的表达与贲门癌临床病理的相关性。方法:采用组织病理学方法分析40例贲门癌的组织病理学诊断并采用免疫组化卵白素-生物素-过氧化物酶复合物法分析其浸润淋巴细胞CD4、CD8、γδT的表达状况和肿瘤细胞P53的表达状况。结果:40例贲门癌组织,P53染色阳性率是75.0%,30例P53阳性贲门癌组织中,P53阳性肿瘤细胞数为99±22。CD8和γδT阳性淋巴细胞数在P53阳性组明显高于P53阴性组(P<0.05);而CD4阳性淋巴细胞数在P53阳性组低于P53阴性组(P>0.05);P53阳性肿瘤细胞与CD8阳性淋巴细胞呈正相关(P<0.05)。CD4、CD8、γδT阳性淋巴细胞在不同性别、不同年龄、不同分化、不同分期及有无淋巴结转移的贲门癌浸润淋巴细胞中的表达差异均无显著性(P>0.05)。结论:贲门癌患者中P53蛋白可能作为肿瘤抗原引起以CD8+T淋巴细胞为主的特异性免疫反应,为P53作为贲门癌患者免疫治疗的一个靶点提供了理论依据。贲门癌组织浸润T淋巴细胞亚群类型与贲门癌临床特征(中低分化、肿瘤中晚期、有无淋巴结转移)均无相关性。Objective To study the relationship of the alterations and clinical significance of CD4, CD8, γδT in infiltrating T lymphoeytes and P53 in tumor cells of gastric cardia adenocarcinoma （GCA） from the high- incidence area for GCA in Henan. Methods By using clinieopathological analysis and immunohistocbemical method （ABC） in 40 GCA tissues, CD4, CD8,γδT and P53 expressions were detected. Results In the 40 GCA tissues, the positive rate for P53 protein in tumor cells was 75.0%. In 30 GCA, the number of P53 positive cells was 99 ± 22. The number of CD8±,γδT positive lymphocytes in P53 positive group was significantly higher than in P53 negative group （P 〈 0.05）. In contrast, the number of CD4 positive T cells in P53 positive group was lower than in P53 negative group （P 〉 0.05）. FUrthermore, the P53 positive cells were correlated well with CD8 positive lymphocytes （P 〈 0.005）. The differences of the CD4, CD8, γδT positive number were not similar in male and female GCA group （P 〉 0.05）. Also it was observed different stage group, with and without Nymph node metastasis group, even in moderately- and poorly differentiated group （P 〉 0.05）. Conclusions P53 protein, as one of tumor antigens, may play a role in inducing the specific immune reaction, in which the CD8 positive lymphocytes play a critical role. T lymphoeytes in GCA do not show any significant relationship with age and gender or clinicopathological changes （different degrees of differentiation, TNM staging and lymph node metastasis）.

关 键 词：胃肿瘤 贲门癌 CD4 CD8 ΓΔT P53 河南 

分 类 号：R735.2[医药卫生—肿瘤]