不同生长阶段鹿茸中胶原含量比较及提取条件优化  

Collagen Content of Velvet Antlers at Different Growth Stages and Optimization of Extraction Process

作　　者：赵玉红[1] 金秀明[1] 王珊珊[1] 

机构地区：[1]东北林业大学林学院食品科学系,黑龙江哈尔滨150040

出　　处：《食品科学》2012年第10期67-71,共5页Food Science

基　　金：黑龙江省教育厅科学技术研究指导项目(11553036)

摘　　要：以梅花鹿鹿茸为原料,研究不同生长阶段鹿茸中胶原含量及胶原提取效果并确定适宜提取条件。采用胃蛋白酶法提取胶原,采用Box-Behnken中心组合试验设计优化胶原提取条件。结果表明:二杠茸中胶原含量和得率均高于毛桃茸和三杈茸,分别为65.8mg/g和4.53%;胶原提取适宜工艺条件为提取时间52h、酶用量5%、液料比23:1(mL/g),在此条件下鹿茸胶原得率为5.92%。Velvet antlers were selected as raw materials to extract collagen using enzyme pepsin. Collagen contents at different growth stages were also compared. Results showed that content and the yield of collagen in two branched antler were 65.8 mg/g and 4.53%, respectively, which were significantly higher than three branched antler （P 〈 0.05）. Factors of extraction time, enzyme addition and liquid-solid ratio were chosen to research the effects on yield of collagen and Box-Behnken experiment was designed to optimize the extraction conditions. The results showed that extraction time for 52 h, enzyme addition with 5%, liquid-solid ratio at 23：1 （mL/g） were the optimal conditions, the yield of collagen in two-branched antler was 5.92%.

关 键 词：鹿茸 胶原 提取 优化 

分 类 号：TS201.7[轻工技术与工程—食品科学]