响应曲面法优化大蒜中总酚提取工艺及其抗氧化活性测定  

Response Surface Methodology for Optimization of Extraction Conditions of Total Phenolic Compounds from Garlic and Determination of Antioxidant Activity

作　　者：杜俊娜[1] 陈书霞[1] 程智慧[1] 常燕霞[1] 周静[1] 

机构地区：[1]西北农林科技大学园艺学院,农业部西北地区园艺作物生物学与种质创制重点实验室,陕西杨凌712100

出　　处：《食品科学》2012年第10期72-77,共6页Food Science

基　　金：西北农林科技大学唐仲英育种基金项目(A212020911);西北农林科技大学青年学术骨干支持计划项目(01140303);国家公益性行业(农业)科研专项(200903018-7)

摘　　要：采用响应曲面法对料液比、提取时间、不同体积分数酸化甲醇提取剂3个因素进行优化,以料液比、提取时间、不同体积分数酸化甲醇提取剂为自变量,总酚提取量为响应值,利用Box-Behnken设计原理和响应曲面法,研究各自变量及其交互作用对总酚提取的影响,模拟求得二次多项回归方程的预测模型,并确定料液比1:14(g/mL)、提取时间31min、80%酸化甲醇为提取剂是最佳的提取条件。在此条件下,总酚提取量为0.67mg GAE/g,模型预测值偏差为6.9%,证明所选工艺条件为最佳工艺条件。同时,用5种方法对其抗氧化活性进行测定,结果表明大蒜具有良好的抗氧化活性。Response Surface Methodology （RSM） was used to optimize extraction parameters （including material/liquid ratio, extraction time and concentration of extracting agents） so as to achieve the maximum extraction yield of total phenolic compounds. Studies were carried out on the respective independent variables （material/liquid ratio, extraction time and concentration of extracting agents） and the interactive effects of these three factors on the total phenolic compounds according to Box-Benhnken design. A mathematical quadratic polynomial regression equation reflecting the relationship between the total phenolic com- pounds and the above extraction parameters was established. The optimal extraction parameters were obtained as： material/ liquid ratio 1：14 （g/mL）, extraction time 31 min, and 80% acidification methanol as extracting agent. Under the optimal condition, the total phenolic content was 0.67 mg GAE/g, and the model prediction deviation was 6.9%. Meanwhile, the antioxidant activity was determined with five different methods, and the results showed that garlic had good antioxidant activity.

关 键 词：大蒜 总酚提取量 抗氧化活性 响应曲面法 

分 类 号：S663.4[农业科学—果树学]