基于金钯纳米合金修饰的过氧化氢传感器的研制  

Development of Hydrogen Peroxide Sensor Modified by Gold-Palladium Nano-Alloys

作　　者：朱伟明[1] 梁新义[1,2] 庞广昌[1] 康晓斌[1] 崔兰[3] 

机构地区：[1]天津商业大学天津市食品生物技术重点实验室,天津300134 [2]天津商业大学理学院,天津300134 [3]天津大学材料科学与工程学院,天津300072

出　　处：《食品科学》2012年第10期311-314,共4页Food Science

基　　金：天津市自然科学基金项目(06YFJMJC12600);天津市自然科学基金重点项目(10JCZDJC23600);教育部留学回国人员科研启动基金项目(20071108);"十一五"国家科技支撑计划项目(2006BAD04A00)

摘　　要：研制金钯合金纳米粒子修饰的特异性定量检测食品中过氧化氢残留量的过氧化氢传感器。以比表面积大、生物相容性好、具有优良电催化性能的金钯合金纳米粒子固定辣根过氧化物酶于玻碳电极,制得过氧化氢传感器电极。通过循环伏安法及交流阻抗法表征电极在组装过程中的电化学特性,利用计时电流法对传感器性能进行考察。研究表明:该传感器测定H2O2的线性范围为1×10-7～5×10-3mol/L,检测限为8.0×10-7mol/L,对H2O2具有较好的催化还原活性和良好的检出性能。该传感器制作简单、成本低廉、可重复性强,可用于快速定量检测食品中过氧化氢残留量。A new type of biosensor for detecting hydrogen peroxide in food residues was developed through modification of gold-palladium nano-alloys on the glassy carbon electrode.Due to large surface area,good biocompatibility and excellent catalytic properties of nano particles,the gold-palladium nano-alloy was used to immobilize horseradish peroxidase（HRP） on glassy carbon electrode.The electrochemical properties of electrode surface during each fabrication procedure were characterized by cyclic voltammetry and AC impedance method and the sensor performance of this sensor was evaluated by chronoamperometry.The results revealed that the hydrogen peroxide sensor had a good linear relationship in the range of 1 × 10-7—5 × 10-3mol/L for H2O2and the limit of detection was 8.0×10-7mol/L.It had better catalytic activity and good detection performance for detecting hydrogen peroxide.Moreover,this hydrogen peroxide sensor proved inexpensive,easy to use and strong repeatability,thus it is applicable for the hydrogen peroxide in food residues.

关 键 词：过氧化氢传感器 金钯合金纳米粒子 玻碳电极 计时电流法 

分 类 号：TS207.3[轻工技术与工程—食品科学]