Elf-1在非小细胞肺癌中的表达与肿瘤细胞凋亡及患者预后的关系  

Expression of Elf-1 in non-small cell lung cancer and its correlationship with prognosis of patients and tumor cell apoptosis

作　　者：杨东霞[1] 李乃娥[2] 

机构地区：[1]滨州医学院基础学院病理学教研室,烟台264003 [2]滨州医学院临床学院诊断学教研室,烟台264003

出　　处：《临床与实验病理学杂志》2012年第7期768-771,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨转录因子Elf-1在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达与凋亡抑制蛋白(inhibitorof apoptosis protein,IAP)家族中最强的凋亡抑制因子survivin关系。方法采用免疫组化PV-9000法检测60例NSCLC组织芯片及9例正常肺组织中Elf-1的表达水平;脱氧核苷酸移换酶(terminal deoxynucleotidy transferase,TdT)介导的dUTP切口末端标记方法(TUNEL)检测癌细胞凋亡指数(apoptosis index,AI)。结果在正常肺组织中有1例Elf-1弱阳性,余为阴性,在NSCLC组织中的阳性率为70.0%,其表达水平与肿瘤细胞分化程度、淋巴结转移、临床分期和术后生存期有关(P<0.05)。Kaplan-Meier生存分析表明其过表达与患者的生存率有关,阳性患者的生存率明显低于阴性患者(P<0.01)。Spearman相关分析显示,Elf-1在NSCLC组织中的表达与AI呈负相关(r=-0.708,P<0.01)。结论 Elf-1在NSCLC组织中的过表达影响着肿瘤细胞的凋亡,与肿瘤细胞分化程度、淋巴结转移和预后有关,检测其表达水平可作为判定NSCLC恶性生物学行为的参考指标。Purpose To investigate the expression of Elf-1 in NSCLC and to explore the relationship between expression of Elf-1 with survivin which is the most effective inhibitor of apoptosis protein（IAP） in non-small cell lung cancer （NSCLC）. Methods Power Vi- sion-9000 immunohistochemistry was used to evaluate the expression of Elf-1 in tissue microarray containing 60 specimens of NSCLC and 9 specimens of normal tissue. The apoptotic cells were tested by terminal .deoxynucleotidy transferase（TdT） mediated Dutp-biotin nick end labelling （TUNEL）. Results Elf-1 was detected in 1 of 9 normal tissues with weak positive, the positive rate of Elf-1 was 70% in NSCLC. The expression level of Elf was significantly associated with tumor differentiation, lymph node metastasis, clinical stage, and postoperative survival time （all P 〈0.05 ）. Overexpression of Elf-1 was associated with poor prognosis：the survival rate was significantly lower in positive patients than in negative patients （P 〈0.01）. Elf-1 expression in NSCLC was negatively associated with AI （r=-0.708, P〈0. 01）. Conclusion The results show that Elf-1 expression in NSCLC correlates with decreased apoptosis of tumor cells. Detecting of Elf expression can help to predict the malignant behavior of NSCLC.

关 键 词：肺肿瘤 非小细胞肺癌 ELF-1 凋亡 预后 

分 类 号：R734.2[医药卫生—肿瘤]