rhBMP-2、胶原、珊瑚复合骨诱导骨形成生物活性的实验研究  

The Experimental Study on the Activity of rhBMP-2, Coral and Collagen Composites Inducing Intramuscle Bone

作　　者：马秦[1] 毛天球[1] 刘宝林[1] 戴毅敏[1] 韩亮[1] 

机构地区：[1]第四军医大学口腔医学院,710032

出　　处：《华西口腔医学杂志》2000年第2期94-97,共4页West China Journal of Stomatology

摘　　要：目的 :研究珊瑚、胶原、rhBMP 2复合骨异位诱导新骨形成的活性和载体在体内降解情况。方法 :选择珊瑚和Ⅰ型胶原作为rhBMP 2的载体 ,采用肌袋异位成骨的动物模型 ,通过大体、组织学观察、组织测量等手段 ,研究复合骨诱导新骨形成的特点、珊瑚降解的过程。结果 :rhBMP 2、珊瑚和胶原复合骨诱导骨组织形成 ,在一定范围内随作用时间延长新骨形成量持续增加 ,其中rhBMP 2含量高时 ,早期新骨形成快 ,成骨量大 ,珊瑚对照组中珊瑚降解较其他组快。结论 :rhBMP 2、胶原、珊瑚复合骨具有骨引导性和骨诱导性 ,珊瑚和胶原在体内可降解。Objective:To investigate the ectopic bone induction activity of the composite of coral, collagen and rhBMP-2, and the degradation of the carriers of rhBMP-2.Methods: The composites of rhBMP-2, coral and collagen were implanted into the muscle pouches of mice. The mice were divided into the following four groups. Group H: each composite had 2 mg rhBMP-2, Group L: each composite had 0.5 mg rhBMP-2, Group J: each composite had only type Ⅰ collagen, Group C: only coral was implanted in the muscle of mouse. Each group had 20 mice. Every 5 mice in each group were killed postoperatively after 1,2, 4 and 8 weeks. The ectopic osteoinductive activity was assessed with histology and histomorphometry. The point counting method was applied to measure the formation of new bone and cartilage and the degradation of coral in the composites. The data was analyzed statistically using t test.Results: There was no obvious morbidity in any of the animals in this study, and no wound infection, bleeding, or feeding difficulties postoperatively. The samples were examined histologically. Cartilage and bone induction occurred in Group H and L. After 1 Week, new bone was observed in Group H, and cartilage formation occurred mainly in Group L. After 2 weeks, both cancellous bone and bone marrow elements were present in Group H and L. New bone formation was not uniform throughout the specimen. Part of the coral in the composites was not absorbed between 2 to 4 weeks postoperatively. At the 8th week, the coral in the composite was absorbed completely. While in Group J and C there was not any new cartilage or bone formation. According to the statistical analysis, bone formation occurred 2 weeks earlier in Group H than in Group L after operation. From 4 postoperative weeks, no difference of the bone formation rate was observed between Group H and Group L. The degradation rate of coral in Group C was the fastest among the four groups.Conclusion: The composites of rhBMP 2, coral and collagen are degradable bone substitutes with bone induction a

关 键 词：诱导成骨 人工骨 RHBMP-2 胶原 珊瑚 

分 类 号：R783.1[医药卫生—口腔医学]