间歇性低氧通过上调脂蛋白相关磷脂酶A_2和氧化型低密度脂蛋白表达促进动脉粥样硬化形成  

Intermittent Hypoxia Promote the Formation of Atherosclerosis by Increasing Expression of Lipoprotein-Associated Phospholipase A_2 and Oxidized Low Density Lipoprotein

作　　者：李月春[1] 刘国荣[1] 王宝军[1] 郝喜娃[1] 张京芬[1] 庞江霞[1] 闫洁[1] 

机构地区：[1]包头市中心医院神经内科,内蒙古包头市014040

出　　处：《中国动脉硬化杂志》2012年第9期792-796,共5页Chinese Journal of Arteriosclerosis

摘　　要：目的研究间歇性低氧及高脂饮食通过上调脂蛋白相关磷脂酶A2(Lp-PLA2)、氧化型低密度脂蛋白(ox-LDL)的表达促进动脉粥样硬化的形成。方法采用随机对照、前瞻性动物实验和析因设计的方法,建立间歇性低氧和高脂饮食兔动物模型,将24只4月龄新西兰大耳白兔随机分为四组:对照组、间歇性低氧组(IH组)、高脂饮食组(HFD组)和间歇性低氧+高脂饮食组(IH+HFD组),每组6只。IH组和IH+HFD组置于间歇性低氧舱中,每天8 h,每循环5 min,舱内最低氧浓度8%,最高氧浓度21%,HFD组和IH+HFD组给予高脂饲料饲养,间歇性低氧和高脂饮食干预12周,利用酶联免疫吸附法测定间歇性低氧和高脂饮食干预0、4、8和12周血浆中Lp-PLA2和ox-LDL含量的变化,在实验终点12周取主动脉弓和腹主动脉观察动脉粥样硬化的形成情况。结果在第8周和12周时,IH组、HFD组和IH+HFD组Lp-PLA2含量较对照组和第4周时升高(P<0.05);在第4、8和12周时IH+HFD组Lp-PLA2含量分别较对照组、IH组和HFD组升高(P<0.05);间歇性低氧和高脂饮食分别在第4、8和12周对Lp-PLA2的影响存在交互效应(P=0.000,P=0.001,P=0.000)。在第4、8和12周时,IH组、HFD组和IH+HFD组ox-LDL含量较对照组均升高(P<0.05),在第4周时明显高于第8周和12周(P<0.05);在第4、8和12周时IH+HFD组ox-LDL含量分别较对照组、IH组和HFD组升高(P<0.05);间歇性低氧和高脂饮食在第4周时对ox-LDL的影响存在交互效应(P=0.000),在第8周和12周时不存在交互效应(P=0.104和P=0.166)。间歇性低氧和高脂饮食干预下腹主动脉油红"O"染色及主动脉弓和腹主动脉组织HE染色后可观察到动脉粥样硬化的形成。结论间歇性低氧和高脂饮食可能通过上调血浆中Lp-PLA2和ox-LDL表达促进动脉粥样硬化形成。Aim To investigate the intermittent hypoxia and high-fat diet promoting the formation of atherosclero- sis through increasing expression of lipoprotein-associated phospholipase A2 （ Lp-PLA2 ） and oxidized low density lipoprotein （ox-LDL）. Methods Using randomized controlled study, prospective animal and factorial experiment, the animal mod- els were established by intermittent hypoxia and high-fat diet. 24 New-Zealand white rabbits （4 months old） were divided into the following 4 groups： control group, intermittent hypoxia group（ IH）, high-fat-feeding group（HFD） and intermittent hypoxia ＆ high-fat-feeding group （ [H ＋ HFD）. Each group had 6 rabbits. Rabbits in IH group and IH ＋ HFD group were placed in a cabin which nitrogen and air were periodically infused every 5 minutes for 8 h/day. The lowest level of oxygen concentration was at 8%. The highest level of oxygen concentration was at 21%. The HFD group and IH ＋ HFD group were fed with fat-rich-stoyer. The intervention experiment lasted for 12 weeks. Blood samples for measurements of Lp-PLA2 and ox-LDL were collected at 0 w, 4 w, 8 w and 12 w. The formation of atherosclerosis in the aortic arch and abdominal aorta was observed. Result The Lp-PLA2 level of IH group, HFD group and IH ＋ HFD group at 8th and 12th week was higher than control group and at 4th week, the difference has statistic significance （P 〈 0. 05）. The Lp- PLA2 level of IH ＋ HFD group at 4th, 8th and 12th week was higher than control group, IH group and HFD group（ P 〈 O. 05）. The two factors of IH and HFD had significant synergistic effects on the Lp-PLA2 at 4th, 8th and 12th week（P = 0. 000,P = 0. 001 ,P = 0. 000）. The ox-LDL level of IH group, HFD group and IH ＋ HFD group at 4th, 8th and 12th week was higher than control group（P 〈 0.05）. It was higher at 4th week than at 8th and 12th week （P 〈 0. 05）. The ox-LDL level of IH ＋ HFD group at 4th, 8th and 12th week was higher than control group, IH group and HFD group（ P 〈 0.

关 键 词：间歇性低氧 高脂饮食 脂蛋白相关磷脂酶A2 氧化型低密度脂蛋白 动脉粥样硬化 

分 类 号：R363[医药卫生—病理学]