反义寡核苷酸对血管瘤内皮细胞VEGF表达的影响  

Effect of Antisense Oligonucleotide on Expression of Vascular Endothelial Growth Factor in Cultured Skin Hemangioma Endothelial Cells

作　　者：雷水生[1] 邓镇[2,3,4] 朱晓琴[2] 朱璐[1] 张远红[1] 唐晓丹[1] 孙丹[1] 刘光敏[1] 

机构地区：[1]广州医学院第五附属医院皮肤科 [2]广州医学院生理学教研室 [3]广州医学院基础学院 [4]广州510182

出　　处：《华中科技大学学报（医学版）》2012年第3期341-344,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：广东省医学科研基金(No.A2012286)

摘　　要：目的探讨反义寡核苷酸(AS-ODN)对培养的血管瘤内皮细胞中血管内皮生长因子(vascular endothelialgrowth factor,VEGF)表达的影响。方法将VEGF反义寡核苷酸(AS-ODN)、正义寡核苷酸(S-ODN)和错义寡核苷酸(M-ODN)分别转染至体外培养的皮肤血管瘤内皮细胞中,采用RT-PCR技术检测各组细胞中VEGF mRNA的表达水平;ELISA法检测各组细胞培养上清液中VEGF蛋白分泌的改变。结果 RT-PCR结果显示:AS-ODN组VEGF mR-NA表达水平较空白对照组明显下降,而S-ODN组、M-ODN组与空白对照组比较,其VEGF mRNA表达未见明显改变。ELISA结果显示:AS-ODN组细胞培养上清液中的VEGF蛋白分泌浓度较空白对照组明显下降,而S-ODN组、M-ODN组与空白对照组比较,VEGF浓度未见明显变化。结论 VEGF AS-ODN转染可以有效地抑制血管瘤内皮细胞VEGF mRNA表达,降低VEGF蛋白分泌,从而有效地抑制血管瘤内皮细胞的生长。Objective To investigate the impact of antisense oligonucleotides（AS-ODN）on expression of vascular endothelial growth factor（VEGF）in cultured skin hemangioma endothelial cells.Methods AS-ODN,S-ODN and M-ODN were transfected into skin hemangioma endothelial cells respectively.The expression of VEGF mRNA was detected by using RT-PCR.The secretion change of VEGF protein was detected by using ELISA in culture supernatants of skin hemangioma endothelial cells.Results RT-PCR revealed that the expression level of VEGF mRNA in AS-ODN-treated group was significantly decreased as compared with that in blank control group.There was no significant change in the expression of VEGF mRNA in S-ODN-treated group,M-ODN-treated group and control group.ELISA demonstrated that the secretion of VEGF protein in AS-ODN-treated group was significantly decreased as compared with that in control group,but no obvious changes in the secretion of VEGF were found in S-ODN-treated group,M-ODN-treated group and control group.Conclusion VEGF AS-ODN transfection can effectively inhibit the expression of VEGF mRNA in hemangioma vascular endothelial cells and reduce VEGF protein secretion,which can effectively inhibit the growth of hemangioma vascular endothelial cells.

关 键 词：反义寡核苷酸 血管内皮生长因子 蛋白分泌 

分 类 号：R732.2[医药卫生—肿瘤]