利用乳汁体细胞研究共轭亚油酸钙对奶牛乳腺基因表达的作用  

Effect of CLA-Ca on lipogenic genes expression of the mammary gland in lactating cows by using milk somatic cell

作　　者：韩立强[1] 王月影[1] 王林枫[1] 郭豫杰[1] 鲁维飞[1] 杨国宇 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]农业部动物生化与营养重点实验室,河南郑州450002

出　　处：《中国兽医学报》2012年第7期1033-1037,共5页Chinese Journal of Veterinary Science

基　　金：国家"973"规化资助项目(2011CB100802)

摘　　要：8头泌乳中期的奶牛被随机分为2组,分别是对照组(每头牛:基础日粮+棕榈酸钙200g/d)和试验组(每头牛:基础日粮+共轭亚油酸钙200g/d),试验期14d,检测了奶产量、乳成分,分析奶牛的血液变化,并采用荧光定量PCR对乳汁体细胞中脂蛋白脂酶(LPL)、乙酰辅酶A羧化酶(ACACA)的基因表达进行检测。结果显示,与对照组相比,试验组奶牛的乳产量、乳蛋白、乳糖和乳汁体细胞含量没有显著影响,但是显著降低了乳脂肪含量(P<0.05),对照组和试验组奶牛乳脂肪含量分别为0.032 6g/mL和0.024 4g/mL。检测血液指标发现,共轭亚油酸钙显著升高了奶牛血液中高密度脂蛋白的含量(P<0.05)。基因分析发现,试验组奶牛乳汁体细胞中LPL、ACA-CA的基因表达显著下调,表明共轭亚油酸钙抑制了奶牛乳腺细胞脂肪酸合成酶的基因表达。Eight Holstein cows were randomly divided into two groups and received a dietary fat supplement of either Ca-salts of palm oil fatty acids （control） or Ca-salts of conjugated linoleic acid （CLA） at 200 g/d per capita for 14 d. Milk yield,milk composition and bloood parameters were determined. The gene expression of LPL and ACACA was assessed via real-time PCR. The results indicated that milk yield and the content of protein,lactose and somatic cell in milk were unaffected by treatment. The cows receiving CLA supplement had a low content of milk fat（0. 024 4 g/ mL,P〈0.05）. The content of HDL in blood was significantly increased in the cows received the CLA-Ca（P〈0.05）. The CLA diet also decreased gene expression of lipogenic enzyme of LPL,ACACA in milk somatic cell,indi- cating that CLA may inhibit the gene expression of lipogenic enzyme in mammary gland.

关 键 词：共轭亚油酸 乳脂肪 乳汁体细胞 脂蛋白酯酶 乙酰辅酶A羧化酶 

分 类 号：S852.2[农业科学—基础兽医学]