一种新型凝固剂在固体平板分离极端浸矿菌中应用潜力研究  

Application Potential of a New-type Coagulator for Purifying Bioleaching Microorganisms by the Solid Plate Method

作　　者：夏金兰[1,2] 潘佳民[1] 巩三强[1] 金雪洁[1] 聂珍媛[1] 闫艺赢[1] 邱冠周[1,2] 

机构地区：[1]中南大学资源加工与生物工程学院,湖南长沙410083 [2]生物冶金教育部重点实验室,湖南长沙410083

出　　处：《当代化工》2012年第6期624-628,共5页Contemporary Chemical Industry

摘　　要：利用一种新型、耐高温、耐酸的基质作凝固剂,设计了一种新型的分离纯化极端浸矿菌的固体平板培养基。在此基础上,结合水盘培养法,成功的实现了高温嗜热浸矿菌、中等嗜热浸矿菌以及嗜中温浸矿菌的固体平板培养及纯化。待试验的三种极端浸矿菌:高温嗜热浸矿菌万座酸菌(Acidianus manzaensis)、中等嗜热浸矿菌(Ferroplasma thermophilum L1)、嗜中温浸矿菌(来自实际矿山酸性矿坑水)都能在此固体培养基表面形成圆形粘液状米黄色菌落;菌落扫描电镜结果表明:菌落内部结合较为松散,单个细菌通过胞外附属结构或物质与培养基基质紧密结合。实验结果表明该固体培养基在浸矿菌分离纯化、菌种鉴定、以及遗传改良等方面具有广阔的应用前景。A kind of solid plate culture medium for purifying bioleaching microorganisms was designed by using a new high temperature resistance and acid resistance coagulator. Combined with the water-dish culture method, solid plate culture and purifying of thermophilic bioleaching bacteria（Acidianus manzaensis）, midthermophilic bioleaching bacteria（Ferroplasma thermophilum L1） ,mesophilic bioleaching bacteria （from the natural acid mine drainage） were realized. The scanning electron microscope revealed that the colony inner was incompact, bacteria in the colony tightly adhered to stroma of the solid plate by extraceUular appendages. These results indicate that the new solid plate will have great potential for screening, identification, genetic engineering of leaching bacteria.

关 键 词：凝固剂 固体培养基 浸矿微生物 扫描电镜 

分 类 号：TQ314[化学工程—高聚物工业]