茶树品种及萎凋过程中叶片APX基因表达的qPCR分析  

Quantitative analysis on the expression of APX gene by qPCR in different tea cultivars and in the withering process of the leaves

作　　者：孙云[1,2] 林玉玲[1] 赖钟雄[1,2] 江春柳[1,2] 

机构地区：[1]福建农林大学园艺植物生物工程研究所 [2]福建农林大学园艺学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2012年第4期476-481,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家星火计划项目(S2011C400031);福建省现代农业茶叶产业技术体系专项资金;国家科技支撑计划课题(2007BAD07B02);福建省农业科技平台建设项目(2008N2001)

摘　　要：应用实时荧光定量聚合酶链式反应(qPCR)的方法对不同茶树品种及萎凋过程中抗坏血酸过氧化物酶(APX)基因的表达进行定量分析.结果表明:茶树不同品种APX基因的表达量存在显著差异,相对表达量变化范围为0.65-5.69,6个茶树品种APX基因的相对表达量从大到小依次为毛蟹、福云6号、肉桂、福鼎大白茶、黄旦、铁观音;茶叶APX基因的表达明显受到萎凋工艺的影响,在茶叶萎凋过程中APX基因的表达量呈现"升高—降低—再升高—再降低"的趋势,相对表达量变化范围为0.02-7.46.Expression of ascorbate peroxidase （APX） gene in different tea cultivars and in the withering process of tea leaves was analyzed by fluorescence quantitative polymerase chain reaction （qPCR） technology. It was showed that the expression amounts of /fiX gene in different tea cultivars were significantly different. The changing range of relative expression amounts was from 0.65 to 5.69. The ranking list of relative expression amounts of APX gene in 6 tea cultivars was： Maoxie 〉 Fuyun 6 〉 Rougui 〉 Fudingd- abaieha 〉 Huangdan 〉 Tieguanyin. In the process of tea withering, the expression amounts of APX gene increased at first, followed by a decline, and then increased again, finally decreased, and the change range of relative expression amounts was from 0.02 to 7.46; at the same time, the expression of/fiX gene was affected by the treatment of withering technique.

关 键 词：茶叶 品种 萎凋 抗坏血酸过氧化物酶(刖强)基因 定量表达 

分 类 号：S571.1[农业科学—茶叶生产加工]