Aurora-A高表达对食管癌细胞侵袭和MMP-9表达的影响  

Effect of Aurora-A Overexpression on Invasion and MMP-9 Expression of Esophageal Carcinoma Cells

作　　者：王晓霞[1,2,3] 路娜[1] 刘志荣[1] 解军[1] 王惠珍[1] 牛勃[1] 裴毅[2,3] 

机构地区：[1]山西医科大学生物化学与分子生物学教研室,太原030001 [2]山西医学科学院 [3]山西大医院,太原030032

出　　处：《中国生物工程杂志》2012年第7期26-30,共5页China Biotechnology

基　　金：国家自然科学基金(30973401);山西省自然科学基金(2009011052-1)资助项目

摘　　要：目的:建立Aurora-A高表达的稳定细胞系,并探讨Aurora-A高表达对食管癌细胞侵袭和MMP-9蛋白表达与活性的影响。方法:利用脂质体法稳定转染Aurora-A表达质粒,通过Westernblot和RT-PCR鉴定Aurora-A高表达的稳定细胞系。利用细胞体外侵袭实验观察细胞的侵袭能力,并通过Western blot、ELISA和明胶酶谱的方法观察MMP-9的蛋白表达水平和活性。结果:建立了Aurora-A高表达的稳定细胞系,且Aurora-A高表达能增加食管癌细胞的体外侵袭能力,并提高了MMP-9的蛋白表达水平和活性。结论:初步阐明了Aurora-A促进食管癌细胞侵袭的作用以及分子机制,为Aurora-A可能成为治疗食管癌的有效靶点提供了依据。Objective：To establish Aurora-A overexpressing transfectant clones and investigate its effect on invasiveness as well as protein expression and activity of MMP-9 in esophageal carcinoma cells. Methods：The Aurora-A expressing plasmid was transfected using Lipofectamine？ Llpofectamme 2000 and the identification of transfectedclones was confirmed by Western blot analysis and RT-PCR. The invasive properties of cells were examined by an in vitro Boyden chamber invasion assay. Level of MMP-9 protein expression and activity were measured with Western blot and ELISA as well as gelatin zymography analysis. Results：Aurora-A overexpressing transfectant clone was established, which increased cell invasion as well as protein expression and activity of MMP-9. Conclusion：The elucidation the molecular mechanism for the promotion of invasion in esophageal carcinoma by Aurora-A may lead to the development of new target for effective therapy.

关 键 词：AURORA-A 侵袭 MMP-9 

分 类 号：R734.2[医药卫生—肿瘤]