大麦虫超氧化物歧化酶分离纯化及性质研究  

Purification and Characterization of Superoxide Dismutase from Zophobas morio L. Larvae

作　　者：张建新[1] 刘娜[1] 何桂梅[1] 郭倩[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《食品科学》2012年第11期215-218,共4页Food Science

摘　　要：目的:探讨大麦虫超氧化物歧化酶(SOD)分离纯化条件及其性质,为大麦虫利用提供科学依据。方法:以大麦虫为原料,经盐析沉淀、DEAE-Sepharose FF阴离子交换柱层析和Sephadex G-75凝胶过滤层析等纯化,变性聚丙烯酰胺凝胶电泳(SDS-PAGE)方法对蛋白质的组分进行分离并确定其分子质量,原子吸收光谱仪鉴定酶的类型。结果:获得了一种酶比活力较高的大麦虫SOD,酶的纯化倍数为68.54倍,分子质量为37.30kD,属于含铜锌超氧化物歧化酶(Cu,Zn-SOD),且在278nm波长处有紫外吸收峰,稳定性较好,最适温度为40℃,最适pH值为6.0,对H2O2、β-巯基乙醇试剂十分敏感,受氯仿-乙醇混合液(3:5,V/V)的影响较小。结论:大麦虫SOD具有较高的酶比活力,稳定性好,具有较高的利用价值。Objective： To explore the purification and properties of superoxidae dismutase（SOD） from Zophobas morio L.larvae.Methods： SOD were obtained from Zophobas morio L.larvae by salting-out,DEAE-Sepharose FF column chromatography and Sephadex G-75 column chromatography.SDS-PAGE was used to determine its molecular weight and atomic absorption spectrometer was used to identify it types.Results： SOD with highly specific activity was obtained.The purification factor was 68.54.The purified SOD was identified as Cu,Zn-SOD with molecular weight of 37.30 kD.It had an absorption peak at 278 nm and good stability.Its optimal temperature and pH were 40 ℃ and 6.0,respectively.Its high sensitivity to H2O2 and β-mercaptoethanol was found,while a mixture of CHCl3 and CH3CH2OH（3：5,V/V） had little effect on its activity.Conclusion： The SOD extracted from Zophobas morio L.larvae has high enzymatic activity,stability and application potential.

关 键 词：大麦虫 超氧化物歧化酶 分离纯化 性质 

分 类 号：Q969.481.1[生物学—昆虫学]