不同气体环境对益生菌Bifidobacterium lactis V9生长的影响  

Effects of air condition on the viability of probiotic bacteria:Bifidobacterium lactis V9

作　　者：其木格苏都 白梅[2] 孔亚楠[2] 魏爱彬[2] 王记成[2] 张和平[2] 

机构地区：[1]青岛君益食品有限公司,山东青岛266107 [2]内蒙古农业大学、乳品生物技术与工程教育部重点实验室,内蒙古呼和浩特010018

出　　处：《微生物学通报》2012年第7期931-939,共9页Microbiology China

基　　金：国家863计划项目(No.2011AA100901;2011AA100902)

摘　　要：Bifidobacterium lactis V9(B.lactis V9)是一株具有良好益生特性且遗传稳定的益生菌,工业化生产环境中气体组成关系着益生菌活菌数量,进而影响其益生功效。【目的】研究不同气体环境对B.lactis V9生长及代谢的影响。【方法】在固体MRS培养基、液体MRS培养基及巴氏杀菌脱脂乳接种B.lactis V9,于不同的气体环境中培养。【结果】在固体MRS培养基上,B.lactis V9在混合气体(N2:H2:CO2=80:10:10)环境中菌落形成较氮气环境(N2:99.99%)多,在空气环境(N2:O2≈79:21)中菌落形成极少。B.lactis V9在MRS液体中培养24 h,混合气体环境下其活菌数(9.11±0.11 log CFU/mL)显著高于空气环境下的活菌数(8.04±0.10 log CFU/mL)(P<0.01),在混合气体环境下B.lactis V9代谢生成的乙酸和乳酸量分别为12.79±0.86 mmol/L和11.99±0.73 mmol/L,显著高于在空气环境中生成量0.65±0.07 mmol/L和2.75±0.57 mmol/L(P<0.01),乙酸/乳酸比值分别为1.06:1和0.24:1。B.lactis V9在巴氏杀菌脱脂乳中发酵18 h,混合气体环境下pH值(4.48±0.07)显著低于空气环境下的pH值(5.03±0.12)(P<0.01),混合气体环境下其活菌数(9.02±0.15 log CFU/mL)显著高于空气环境下的活菌数(8.53±0.08 log CFU/mL)(P<0.01)。混合气体和空气环境下发酵脱脂乳产生的乙酸和乳酸量分别为60.52±2.30 mmol/L、5.17±1.02 mmol/L和16.86±0.34 mmol/L、5.92±0.81 mmol/L,乙酸/乳酸的值分别为11.71:1和2.85:1。【结论】在N2:H2:CO2=80:10:10混合气体环境下有利于B.lactis V9在液体MRS和脱脂乳中生长,其活菌数可以增加0.5-1个数量级。这一研究结果也可为B.lactis V9益生菌发酵乳的生产和产品中B.lactis V9活菌培养计数提供指导。Bifidobacterium lactis V9 （B. lactis V9） has been demostrated as a probiotic with well properties and stable genetics. The gas composition in environment of industrial manufacturation is positively associated with viable numbers of probiotic and nextly have influence on probiotic properties. [Objective] The influence of different gas environment on the growing of B. lactis V9. [Methods] By MRS agar plate, liquid MRS medium and pasteurized skim milk inoculated with B. lactis V9. [Results] On the MRS agar plate, the numbers ofB. lactis V9 forming colonies under mixed gas environment （N2：H2：CO2=80：10：10） was more than nitrogen gas environment （N2： 99.99%） and few colonies formed under normal air environment （N2：O2≈79：21）. In the MRS liquid medium for 24 h, the viable counts under mixed gas were 9.11±0.11 log CFU/mL which was significantly higher than growing in normal air condition with 8.04±0.10 log CFU/mL （P〈0.01）. Moreover, the acetate and lactate production under mixed gas condition were respectively significantly higher than normal air condition （12.79±0.86 mmol/L VS 11.99±0.73 mmol/L, 0.65±0.07 mmol/L VS 2.75±0.57 mmol/L, P〈0.01）. In addition, the acetate/lactate molar ratio was 1.06：1 and 0.24：1, respectively. After 18 h of pasteurized skim milk fermentation, the reduced pH of fermented milk under mixed gas condition was significantly lower than normal air condition （4.48±0.07 VS 5.03±0.12, P〈0.01）. The viable counts under mixed gas were 9.02±0.15 log CFU/mL which was significantly higher than growing in normal air condition with 8.53±0.08 log CFU/mL （P〈0.01）. The acetate and lactate production under mixed gas condition vs. normal air condition in fermented milk were 60.52±2.30 mmol/L and 5.17±1.02 mmol/L VS 16.86±0.34 mmol/L and 5.92±0.81 mmol/L, repectively. The acetate/lactate molar ratio was 11.71：1 and 2.85：1, repectively. [Conclusion] These study suggested that mixed gas condition with N2：H2：CO2=80：10：10 was beneficial

关 键 词：益生菌 BIFIDOBACTERIUM LACTIS V9 气体组成 生长 

分 类 号：R371[医药卫生—病原生物学]