响应曲面优化超声辅助提取山茱萸籽油工艺及其成分分析  

Optimization of Ultrasound-Assisted Extraction of Cornus officinalis Seed Oil by Response Surface Methodology and Chemical Composition Analysis

作　　者：刘瑞林[1] 詹汉英[1] 陈钿[1] 牟朝丽[1] 贾晓萌[1] 张志琪[1] 

机构地区：[1]陕西师范大学化学化工学院,药用资源与天然药物化学教育部重点实验室,陕西西安710062

出　　处：《食品科学》2012年第12期1-7,共7页Food Science

基　　金：国家自然科学基金面上项目(20875060);陕西师范大学2012年学生暑期社会实践项目

摘　　要：研究以正己烷为溶剂超声辅助提取山茱萸籽油的工艺,并对其成分进行分析。采用响应曲面优化法(RSM)优化山茱萸籽油的提取工艺,确定最佳提取工艺参数为提取温度40℃、提取时间40min、液料比10:1(mL/g),在此条件下,山茱萸籽油的一次提取率为97.08%(出油率7.90%)。利用GC-MS对山茱萸籽油中脂肪酸成分进行分析,结果表明:山茱萸籽油的主要脂肪酸成分为油酸(61.89%)、硬脂酸(19.99%)、棕榈酸(7.63%)和亚油酸(6.33%)等,不饱和脂肪酸占脂肪酸总量的69.45%;采用高效液相色谱法测定,山茱萸籽油中VE含量为76.0mg/100g。Abstract： Response surface methdology was employed to optimize the ultrasound-assisted extraction of Comus officinalis seed oil. Meanwhile, its chemical composition was analyzed by GC-MS. The optimal extraction conditions were determined as 40 ℃ of extracion temperatrure, 40 rain of extraction time, and 10：1 of liquid-to-solid raio （mL/g）. Under these conditions, the extraction yield of Comus officinalis seed oil based on the dry weight of Comus officinalis kernels was 7.90%, accounting for 97.08% of that obtained from Soxhlet extraction. The predominant fatty acids in Comus officinalis seed oil were oleic acid （61.89%）, stearic acid （19.99%）, palmitic acid （7.63%）, linoleic acids （6.33%）, and so on. The percentage of unsaturated fatty acids relative to total fatty acids was 69.45%. In addition, the vitamin E content in Comus officinalis seed oil, as determined by HPLC, was 76.0 mg/100 g.

关 键 词：超声辅助提取 山茱萸籽油 响应曲面法(RSM) 成分分析 

分 类 号：TS224.4[轻工技术与工程—粮食、油脂及植物蛋白工程]