正交试验优化超声辅助法提取三七有效成分工艺  

Orthogonal Array Optimization of Ultrasonic-Assisted Extraction of Panax notoginseng Saponin Rg1

作　　者：周国燕[1] 王春霞[1] 桑迎迎[1] 胡晓亮[1] 

机构地区：[1]上海理工大学低温生物与食品冷冻研究所,上海200093

出　　处：《食品科学》2012年第12期8-11,共4页Food Science

基　　金：国家自然科学基金青年科学基金项目(50206013);上海市教委科研创新项目(09YZ230);上海市重点学科建设项目(S30503);上海理工大学医疗器械与食品学院微创励志创新基金项目

摘　　要：研究超声辅助法提取三七有效成分的工艺。通过单因素试验研究乙醇溶液体积分数、料液比、超声波频率和提取时间对三七人参皂苷Rg1提取率的影响。在单因素试验基础上对4个因素安排L9(34)正交试验,得出影响三七有效成分人参皂苷Rg1提取率的先后次序为:乙醇体积分数>提取时间>料液比>超声频率。选出最佳提取工艺为:乙醇体积分数70%、料液比1:12(g/mL)、超声频率59kHz、提取时间60min。在此最佳工艺下,得到三七干燥后的人参皂苷Rg1含量为1.85%。The ultrasonic-assisted extraction of Panax notoginseng saponin Rgl was optimized using an L9（34） orthogonal array design based on one-factor-at-a-time experiments. The effects of ethanol concentration, material-to-liquid ratio, ultrasonic frequency and extraction time on extraction efficiency were examined. Ethanol concentration had the highest effect on extraction efficiency, followed by extraction time and material-to-liquid ratio; the effect of ultrasonic frequency was the weakest. The optimal levels of ethanol concentraion, material-to-liquid ratio （ghnL）, ultrasonic frequency and extraction time were 70%, 1：12, 59 kHz and 60 min, respectively. Under the optimized extraction conditions, the extraction yield of saponin from Radix Panax notoginseng was 1.85%.

关 键 词：三七 超声辅助法 人参皂苷RG1 提取 

分 类 号：TS205.7[轻工技术与工程—食品科学]