含CpG寡核苷酸抗血管生成作用的实验研究  

Empirical study on anti-angiogenesis effects of CpG oligonucleotide

作　　者：颜伟[1] 孙梯业 杨春敏[1] 贾敏[1] 李静[1] 唐和兰[1] 杜斌[1] 韩全利[1] 杨香丽 

机构地区：[1]解放军空军总医院干部病房,北京100142 [2]解放军第二炮兵总医院肝胆胃肠病研究所 [3]解放军总装备部后勤部亚运村门诊部

出　　处：《西南国防医药》2012年第8期813-816,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的观察CpG ODN对裸鼠肺癌皮下种植瘤血管生成的作用。方法建立裸鼠皮下种植瘤模型,随机分为4组:A组[CpG ODN1826(1μg/μl)+β射线(RT:8 Gy)]、B组[CpG ODN1826(1μg/μl)]、C组[生理盐水(100μl/只)+β射线(RT:8 Gy)]、D组[生理盐水(100μl/只)]。接种肺癌细胞7 d后,开始瘤内注射药物并照射5 d。RT-PCR法测定瘤组织中VEGF-C mRNA、NRP-1 mRNA的表达。结果 4组裸鼠肿瘤组织中VEGF-C mRNA相对表达水平分别为18.34±3.19、29.62±3.14、51.13±2.81、53.46±5.67;NRP-1 mRNA相对表达水平分别为23.57±5.73、34.72±5.13、59.95±4.76、62.49±6.34,A组较其他3组显著下降(P<0.01),B组较C组和D组也显著下降(P<0.01)。结论 CpG ODN1826可显著抑制血管生成,其作用机制可能与抑制VEGF-C和NRP-1的表达有关。Objective To observe the effects of CpG oligonucleotide （ODN） on the angiogenesis of subcutaneous implanted tumor of lung carcinoma in the nude mice. Methods The models of subcutaneous implanted tumor in nude mice was established and randomly divided into 4 groups：group A receiving radiation and medical administration[ CpG ODN 1826（ 1 μg/μl） ± β ray（ RT：8 Gy） ], group B receiving simple medical administration [ CpG ODN 1826 （1μg/μl）], group C receiving irradiation [ saline （ 100 μl/one） ± 13 ray（ RT：8 Gy） ] and group D[ saline（ 100 μl/one） ]. Seven days after the implantation of lung cancer ceU,all those groups received the intratumoral injection of medicine and irradiation for 5 d. The expression of VEGF - C mRNA and NRP - 1 mRNA in the tumor tissue was detected by RT - PCR. Results The relative expression levels of VEGF - C mRNA in the tumor tissue of the four groups above were respectively 18.34± 3.19,29.62 ± 3.14,51.13 ± 2.81 and 53.46 ± 5.67. The relative expression levels of NRP - 1 mRNA in those groups were respectively 23.57 ~ 5.73,34.72 ± 5.13,59.95 ± 4.76 and 62.49 ± 6.34. The expression level of group A decreased very significantly compared with those of the other three groups（P 〈 0.01 ）. Compared with the expression levels of group C and group D,that of group B decreased very significantly（P 〈 0.01 ）. Conclusion CpG ODN1826 can remarkably inhibit the angiogenesis of nude mice tumor,and its mechanism may be correlated with the inhibition of VEGF - C and NRP - 1 expression.

关 键 词：CPG ODN VEGF-C NRP-1 抗血管生成 肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]