遗传性视网膜变性视细胞凋亡与p53基因表达  

Photoreceptor apoptosis and p53 gene expression in inherited retinal degeneration of RSC rats

作　　者：刘斌[1] 朱秀安[1] 

机构地区：[1]北京医科大学第三医院眼科,100083

出　　处：《中华眼科杂志》2000年第1期65-68,I009,共5页Chinese Journal of Ophthalmology

摘　　要：目的　研究遗传性视网膜变性视细胞凋亡的基因调控。方法　对出生后第 13～ 6 0天的遗传性视网膜变性动物模型RCS(RoyalCollegeofSurgeons)大鼠视网膜 ,进行末端脱氧核糖核苷酸连接酶介导生物素dUTP末端标记 (TUNEL)凋亡细胞检测、p5 3蛋白免疫组织化学染色、p5 3mRNA原位杂交和RT PCR。结果　RCS大鼠视网膜视细胞自生后第 2 5天出现凋亡 ,第 30～ 35天达高峰 ;视细胞凋亡初期 ,即生后第 2 8～ 30天 ,视细胞内p5 3蛋白和mRNA水平均显著升高。RT PCR亦显示 ,RCS大鼠视网膜在生后第 2 5～ 35天时可见较多p5 3mRNA扩增产物。结论　遗传性视网膜变性视细胞凋亡初期p5 3基因呈高表达 ,提示p5 3基因可能参与视细胞凋亡的调控。Objective To investigate the gene regulation of photoreceptor apoptosis in inherited retinal degeneration Method The retinas of inherited retinal degeneration in animal models of RCS (Royal College of Surgeons) rats, ranging from 13 th to 60 th postnatal days in age, were studied by TdT mediated biotin dUTP nickend labeling (TUNEL) for apoptosis, immunohistochemistry for p53 protein, in situ hybridization and reverse transcription polymerase chain reaction (RT PCR) for p53 mRNA Results The photoreceptor of RCS rats underwent apoptosis from 25 th postnatal day, and reached the highest level at 30-35 th postnatal days At the early stage of photoreceptor apoptosis, the level of p53 protein and mRNA in photoreceptors increased significantly RT PCR also showed that there was more production of p53 mRNA at 25 to 35 th postnatal days in the retinas of RCS rats Conclusion P53 gene expression increases significantly at the early stage of photoreceptor apoptosis, indicating that p53 gene might play a role in the regulation of photoreceptor apoptosis in inherited retinal degeneration

关 键 词：视网膜变性 视细胞凋亡 P53基因 遗传性 

分 类 号：R774.13[医药卫生—眼科]