人食管癌细胞核基质的研究  

THE NUCLEAR MATRIX IN HUMAN ESOPHAGEAL CARCINOMA CELLS

作　　者：陈海滨[1] 邱殷庆[2] 张锦堃[1] 陈玲[1] 任显辉[2] 杨蕾[2] 

机构地区：[1]汕头大学医学院组织学胚胎学教研室,汕头515031 [2]香港中文大学解剖学系

出　　处：《解剖学报》2000年第1期65-68,I013,共5页Acta Anatomica Sinica

基　　金：李嘉诚先生人才基金;广东省医学科研基金! ( B19970 83 ;B19980 83 )

摘　　要：目的　研究人食管癌细胞株 EC1和 EC18的核基质形态及蛋白成分。　方法　应用细胞的选择性抽提、DGD包埋 -去包埋剂电镜制样观察核基质形态 ,SDS-聚丙烯酰胺凝胶电泳、双向电泳和免疫印迹等分析核基质蛋白成分。　结果　抽提后 ,两种细胞内均存在精细的核基质 -核纤层 -中间丝网架。低分化的 EC1细胞核基质较高分化的 EC18更细密。核基质蛋白电泳显示 ,两株细胞有数种相同的核基质蛋白成分 ,也有各自的特异性核基质蛋白。在免疫印迹分析中 ,使用抗人 Nu MA单克隆抗体检测到两株食管癌细胞中都存在 Nu MA蛋白。　结论　在食管癌细胞中 ,存在特异的核基质蛋白 ,核基质 -核纤层 -中间丝形成一个连续的体系 ,对维持细胞核的完整性和细胞功能起重要作用。Objective\ To investigate the nuclear matrix in two human esophageal carcinoma cell lines EC1 and EC18.\ Methods\ The fine structures of nuclear matrix were observed by DGD(diethylene glycol distearate) embeddment\|free sections and the electrophoretic pattern of the nuclear matrix proteins was compared by SDS\|PAGE,two\|dimensional PAGE and Western blot analysis.\ Results\ The nuclear matrix\|lamina\|intermediate filament fine network remained after selective extraction.The nuclear matrix of the less differentiated EC1 cells was much thinner and denser than that of the more differentiated EC18.Both cell lines shared myriad common nuclear matrix proteins,but cell line specific nuclear matrix proteins was detected.Western blot analysis with anti\|NuMA revealed that nuclear mitotic apparatus protein presented in nuclear matrix proteins of two cell lines.\ Conclusion There are esophageal carcinoma cell specific nuclear matrix proteins and the nuclear matrix\|lamina\|intermediate filament forms a continuous system which plays an important role in the maintenance of the nuclear integrity and cellular function.

关 键 词：核基质 形态学 食管肿瘤 肿瘤细胞 

分 类 号：R735.102[医药卫生—肿瘤]