三磷酸肌醇和PTEN基因表达在槲皮素抑制裸鼠肝癌增长中的作用  

Inositol triphosphate and PTEN gene expression in the quercetin inhibition of liver cancer growth in nude mice

作　　者：彭伟 张继红[2] 梁力建[2] 

机构地区：[1]广州市南沙区中医医院外科,广东广州511462 [2]中山大学附属第一医院肝胆外科,广东广州510080

出　　处：《中国现代医生》2012年第21期8-10,共3页China Modern Doctor

基　　金：广东省广州市南沙区科技项目资助(RG2009009)

摘　　要：目的探讨三磷酸肌醇(IP3)和PTEN基因表达变化在槲皮素(quercetin)治疗裸鼠移植人肝癌中的作用。方法以裸鼠移植人肝癌为对照组,槲皮素治疗组腹腔注入槲皮素50 mg/(kg.d),对照组腹腔注入含0.04%DMSO的RPMI1640培养基0.05 mL/g,3周后观察肝癌增长情况,并应用同位素试剂盒检测肝癌组织IP3含量,RT-PCR分析癌组织PTEN mRNA表达,Western blotting分析肝癌组织PTEN蛋白表达。结果治疗组肝癌体积和重量均显著低于对照组[体积(15.8±10.1)mm3 vs(52.3±26.5)mm3,重量(44.8±10.4)mg vs(91.3±31.4)mg],PTEN mRNA表达显著高于对照组[RI(灰度与面积之积的相对强度)0.81±0.24 vs 0.36±0.09](P<0.01),PTEN蛋白表达显著高于对照组[RI(灰度与面积之积的相对强度)3.14±0.13 vs 1.08±0.15]。结论槲皮素能减少IP3生成,上调肝癌组织PTEN基因表达,抑制裸鼠移植人肝癌增长。Objective To investigate the inositol triphosphate （IP3） and PTEN gene expression changes in the quercetin treatment of nude mice of human hepatoma. Methods The nude mice with transplanted human hepatocellular carcinoma were set as control group, received intraperitoneal injection of RPMI1640 medium containing 0.04% DMSO of 0.05 mL/g, and treatment group received intraperitonea] injection of quercetin 50 mg/（kg·d）, after three weeks the observation of liver cancer growth and HCC tissues IP3 content by application of isotope detection kit, RT-PCR analysis of cancer tissue ex- pression of PTEN mRNA, Western blotting analysis of HCC tissue of PTEN protein expression were carried out. Results The tumor volume and weight of the treatment group were significantly lower than the control group （volume 15.8±10.1 mm3 vs. 52.3±26.5 mm3 and weight 44.8±10.4 mg vs 91.3±31.4 rag）, PTEN mRNA expression was significantly higher than that of the control group [RI gray-scale and area of the plot of relative intensity 0.81±0.24 vs. 0.36±0.09 （P 〈 0.01）], PTEN protein expression was significantly higher than that in the control group [relative intensity RI （grayscale with the area of product） 3.14±0.13 vs. 1.08±0.15]. Conclusion Quereetin can reduce the production of IP3 and increase the gene expres- sion of PTEN in hepatoma tissues, inhibit the growth of nude mice with transplanted human liver cancer.

关 键 词：肝细胞癌 染料木黄酮 PTEN基因 

分 类 号：R735.7[医药卫生—肿瘤]