Calcineurin,NFAT2,COX-2和VEGF-A在体外培养正常黑素细胞和不同黑素瘤细胞系中的表达  

The Expression of Calcineurin,NFAT2,COX-2 and VEGF-A in Normal Melanocytes and Melanoma Cells Cultured in Vitro

作　　者：徐玉荣[1] 廖文俊[1] 蔡越[2] 简强[1] 任婧[1] 刘启方[1] 

机构地区：[1]第四军医大学西京皮肤医院,陕西西安710032 [2]第四军医大学航空航天生理学教研室,陕西西安710032

出　　处：《中国皮肤性病学杂志》2012年第8期667-669,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金(81072235)

摘　　要：目的检测Calcineurin/NFAT信号途径中Calcineurin,NFAT2,COX-2和VEGF-A分子在正常人黑素细胞和不同黑素瘤细胞系中的表达,初步探讨它们与黑素瘤发生、发展的关系。方法选取正常人原代黑素细胞(MC)及来源于原位(WM793B)、侵袭性(LIBR)及转移性(SK-MEL-5)的黑素瘤细胞系,采用RT-PCR,Westernblot检测四种细胞中Calcineurin,NFAT2,COX-2和VEGF-A的表达情况。结果与正常人黑素细胞相比,在不同黑素瘤细胞系中Calcineurin,NFAT2,COX-2和VEGF-A4个分子明显高表达。结论 Calcineurin/NFAT信号途径可能与黑素瘤的发生有关。Objective To detect the level of Calcineurin, NFAT2, COX-2 and VEGF-A in Calcineurin/NFAT signaling pathway in normal melanocytes and melanoma cells. To explore the relationship between Caleineurin/NFAT signaling pathway and the occurrence and development of melanoma. Methods The expression of Calci- neurin,NFAT2, COX-2 and VEGF-A was detected by using RT-PCR and Western blot method in primarily cultured melanoeytes from healthy persons and melanoma cell lines WM793B, LIBR, SK-MEL-5. Results The level of Calcineurin, NFAT2, COX-2 and VEGF-A were increased significantly in melanoma cell lines as compared with normal melanoeytes. Conclusion Caleineurin/NFAT signaling pathway may contribute to the occurrence of melanoma.

关 键 词：黑素细胞 黑素瘤 CALCINEURIN NFAT2 COX-2 VEGF-A 

分 类 号：R739.5[医药卫生—肿瘤]