PCR-SSCP法检测非小细胞肺癌p53基因的突变  

Detection of p53 Gene Mutations in Non small Cell Lung Cancer by PCR SSCP Analysis

作　　者：郑颂国[1] 张容轩 周佰年 罗建民[1] 沈兆忠[1] 许良中[1] 

机构地区：[1]上海医科大学肿瘤医院,200032 [2]上海市第一肺科医院,200433

出　　处：《实用癌症杂志》2000年第1期4-6,共3页The Practical Journal of Cancer

摘　　要：目的　分析原发性非小细胞肺癌（ＮＳＣＬＣ） 中ｐ５３ 基因第５ ～８ 外显子点突变发生及其意义。方法　运用聚合酶链反应－ 单链构象多态性分析方法（ＰＣＲＳＳＣＰ 分析法） 进行检测。结果　发现８ 例肺良性疾病均无点突变发生，４０ 例ＮＳＣＬＣ 中１９例（４７ ．５ ％ ） 有点突变发生，点突变发生与性别，年龄，组织学类型和组织学分级无明显相关性（ Ｐ＞ ０ ．０５） ，但与临床分期和淋巴结累及状态明显相关（ Ｐ＜ ０ ．０１ ，Ｐ＜ ０ ．０５） 。结论　结果表明ｐ５３ 基因点突变在ＮＳＣＬＣ 的发生和进展中起着相当重要的作用。Objective To investigate the significance of p53 gene mutations in NSCLC,with focusing on the four highly conserved regions of the p53 gene.Methods Point mutations of the p53 gene were evaluated by polymerase chain reaction single strand conformation polymorphism(PCR SSCP) analysis in 40 cases of NSCLC and 8 cases of lung benign diseases.Results There was no p53 gene mutation found in 8 cases of lung benign disease,while it was found in 19 of 40 NSCLC samples(47.5%).There was no significant correlation in the frequency of mutations with respect to age,sex,histological subtypes and grades.The frequency of p53 gene mutatatons varied among different clinical stages( P <0.05),with or without nodal involvement( P <0.02).Conclusion These results showed that p53 gene mutations may be associated with the carcinogenesis and progression of NSCLC.

关 键 词：非小细胞肺癌 P53基因 点突变 肺癌 PCR-SSCP 

分 类 号：R734.2[医药卫生—肿瘤]