甘蔗渣和糖蜜混合发酵制备燃料丁醇  

Production of butanol from bagasse and molasses by mixed fermentation

作　　者：杜腾飞[1] 郭亭[1,2] 汤艳[1] 贺爱永[1] 姜岷[1] 

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,南京210009 [2]广州甘蔗糖业研究所,广州510316

出　　处：《生物加工过程》2012年第4期12-15,共4页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)资助项目(2011CB707405);材料化学工程国家重点实验室基金资助项目;江苏省"青蓝工程"资助项目;林业公益性专项基金资助项目(201004001);国家自然科学基金青年基金资助项目(21106067)

摘　　要：以抗逆突变株Clostridium beijerinckii IB4为研究对象,葡萄糖为C源,对其进行补料分批发酵过程的优化,同时将该优化工艺应用于甘蔗渣和糖蜜混合发酵制备燃料丁醇。结果表明:在5 L发酵罐中,先加入作为还原糖的甘蔗渣酸解糖液10 g/L,16 h后补加甘蔗糖蜜30 g/L,于35℃、100 r/min发酵50 h,丁醇和总溶剂产量分别达到11.1和15.3 g/L,丁醇比例高达72.5%。A tolerance mutant strain Clostridium beijerinckii IB4 was selected.Fed-batch fermentations were carried out in P2 fermentation medium with glucose,and the optimal fed-batch fermentation strategy was applied to butanol mixed fermentation from bagasse and molasses.Clostridium beijerinckii IB4 was cultured in a 5 L fermentor at 35 ℃,100 r/min for 50 h.The initial 10 g/L reducing sugar of acid hydrolysate of bagasse was added,and 30 g/L of molasses was fed into fermentation medium after 16 h.Butanol and total solvents concentrations accumulated in the broth were 11.1 and 15.3 g/L,respectively.Butanol proportion was up to 72.5%.

关 键 词：丁醇 CLOSTRIDIUM beijerinckii 补料分批 发酵 混合发酵 

分 类 号：TQ921[轻工技术与工程—发酵工程]