蚕蛹油的提取及其不饱和脂肪酸分离研究  

Silk Worm Pupa Oil Extraction and Separation of Unsaturated Fatty Acids

作　　者：缪丽华[1] 胡晓露[1] 李佑贵 潘海朋[1] 

机构地区：[1]华南农业大学食品学院,广州510642 [2]广州市番禺粮食储备有限公司油脂分公司,广州511480

出　　处：《山东食品发酵》2012年第2期13-16,共4页Shandong Food Ferment

摘　　要：以蚕蛹为原料,采用索氏抽提法从蚕蛹中浸提蚕蛹油,分别采用几种不同的提取溶剂浸出,当在石油醚和丙酮以体积比为1:1,按固液比为1:3时,在65℃条件下回流浸提6小时,得油率高达29.3%。蚕蛹油用碱性液进行提取,经减压浓缩去除溶剂制得混合脂肪酸,再采用尿素包合法提取蚕蛹油中不饱和脂肪酸。最终得出分离不饱和脂肪酸的最佳工艺条件:以甲醇为溶剂,尿素与脂肪酸质量之比为13:1,包合温度为0℃,时间为12小时,可聚合较高纯度的不饱和脂肪酸。Take silkworm chrysalis as raw materials,extract oil from silkworm chrysalis in soxhlet extraction method, respectively, using several different extraction solvents extract oil, while petroleum ether and acetone mixed in volume ratio of 1：1, the solid-liquid ratio of 1：3 ,refluxed in 6 hours at 65℃, and the extraction oil rate as high as 29.3%.Silkworm chrysalis oil extracted with alkaline liquid extraction,and concentrated under vacuum to remove the solvent system from mixed fatty acids, extract unsaturated fatty acids from silkworm chrysalis oil in urea inclusion method. At last,the optimum conditions of separate unsaturated fatty acids：take methanol as solvent, urea and fatty acid mixed as quality ratio of 13：1,at the temperature of 0℃ in 12 hours,and aggregate the higher purity of unsaturated fatty acids.

关 键 词：蚕蛹油 索氏抽提 不饱和脂肪酸 尿素包合 

分 类 号：TS225.2[轻工技术与工程—粮食、油脂及植物蛋白工程]