塞来昔布对大鼠大肠癌生长的抑制作用  

Inhibitory effects of celecoxib on growth of colorectal cancer in experimental rats

作　　者：王伟杰[1] 李海[1] 杜勇[2] 张东[1] 杨银学[1] 

机构地区：[1]宁夏医科大学总医院结直肠外科,银川750004 [2]宁夏医科大学总医院外科学研究室,银川750004

出　　处：《中华实验外科杂志》2012年第7期1328-1330,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然料学基金资助项日（30960370）;宁夏自然科学基金资助项目（NZ10155）

摘　　要：目的观察选择性环氧合酶（COX-2）抑制剂塞来昔布对二甲肼（DMH）诱导的sD大鼠大肠癌生长的抑制作用。方法健康雄性SD大鼠100只，随机分为4组：空白对照组（20只），造模组（30只），干预组（30只）及干预对照组（20只）。空白对照组：皮下注射与造模组DMH等体积的生理盐水，每周1次；造模组：利用大鼠大腿内侧（双后肢交替使用）皮下注射DMH（每周20mg／kg）；干预组：DMH皮下注射量同造模组，同时给予塞来昔布（20mg／kg）灌胃，隔天1次；干预对照组：给予与干预组等体积的塞来昔布灌胃，隔天1次，持续25周。各组于第15、20、25周分别处死大鼠2只，行苏木素一伊红（HE）染色，光镜下观察病理变化，剩余大鼠第30周全部处死。结果实验30周后，空白对照组和干预对照组大鼠大肠未见肿瘤形成，造模组大鼠成瘤率为89．47％，干预组大鼠成瘤率为56．52％，两组差异有统计学意义（P〈0．05）；造模组大鼠成癌率为68．42％，干预组大鼠成癌率为34．78％，两组差异有统计学意义（P〈0．05）；造模组单个大鼠大肠产生的肿瘤数和单个肿瘤直径分别为（2．64±0．86）个和（4．06±1．14）mm；干预组单个大鼠大肠产生的肿瘤数和单个肿瘤直径分别为（1．85±0．80）个和（3．08±0．86）mm，两组差异有统计学意义（P〈0．05）。结论选择性COX-2抑制剂塞来昔布对DMH诱导的SD大鼠大肠癌生长有抑制作用。Objective To investigate whether selective cyclooxygenase-2 inhibitor celecoxib can prevent SD rat colorectal cancer growth induced by l, 2-dimethylhydrazine （DMH）. Methods One hun- dred male Sprague-Dawley rats were randomly divided into four groups： the model group and interferential group were treated with DMH （20 mg/kg/week） to induce colorectal cancer; interferential group was fed on celecoxib once every other day, and the control group was subcutaneously injected with equivalent saline as model group; the intervention control group was fed on equivalent celecoxib as interferential group. All animals received treatment for 25 weeks. Two rats were killed in batches at the 15th, 20th and 25th week of the experiment separately, and then hematoxylin-eosin staining and pathological changes were observed under a light microscope. All remaining rats were killed after 30 weeks. Results Thirty weeks later, the control group and intervention control group had no tumor formation. The colorectal tumor rate of model group and interferential group was 89. 47% and 56. 52% respectively （P 〈 0. 05 ） , and that of model group and interferential group was 68.42% and 34. 78% respectively （ P 〈 0. 05 ）. The number of tumor cells in model group and interferential group was （2. 64 ± 0. 86） and （ 1.85 ± 0. 80） respectively （ P 〈 0. 05 ） ; the diameter of tumor in model group and interferential group was （4. 06 ± 1.14） mm and （3.08 ±0. 86） mm respectively （ P 〈 0. 05 ）. Conclusion Celecoxib, a selective cyclooxygenase-2 inhibitor, may have preventive action against rat colorectal cancer growth induced by DMH.

关 键 词：二甲肼 大肠癌 塞来昔布 

分 类 号：R735.34[医药卫生—肿瘤]