聚丁二酸丁二醇酯生物降解酶及其催化性能研究  

Purification and Characterization of Poly(Butylenes Succinate) Degrading Enzyme

作　　者：李成涛[1] 张敏[1] 白清友[1] 刘武周[1] 王天瑜[1] 宋吉青[2] 

机构地区：[1]陕西科技大学教育部轻化工助剂化学与技术重点实验室,陕西西安710021 [2]中国农业科学院农业部农业环境与气候变化重点开放实验室,北京100081

出　　处：《环境科学与技术》2012年第8期37-40,55,共5页Environmental Science & Technology

基　　金：国家自然科学基金(21144008);陕西省重大科技创新项目(2009ZKC08-09);陕西科技大学科研创新团队资助项目(TD10-01)

摘　　要：聚丁二酸丁二醇酯（PBS）在微生物存在条件下，可降解为低分子量的小分子。文章以实验室自行分离的、能有效降解PBS的菌株ZM-P1为出发菌株进行PBS的降解研究，对降解液中的解聚酶进行分离纯化，研究其生化性质及催化性能。研究结果表明：所得蛋白经过SDS—PAGE电泳鉴定为脂肪酶，该脂肪酶最适温度为60℃，在30—60℃内趋于稳定；其最适pn为9．0，pH6-9范围内酶的活性稳定；Mg2＋该脂肪酶有较强的促进作用，Zn2＋和Na＋对该脂肪酶影响较小，Ca2＋和EDTA对其有明显抑制作用。降解后PBS数均分子量与重均分子量下降，结晶颗粒减小，热稳定也有所下降，说明该菌株对PBS有较为明显的降解效果。Polybutylenes succinate （PBS）can be degraded into oligomers by microorganism. The strain ZM-P1 separated by the laboratory was used for PBS degradation experiment, the depolymerization were separated and purified to study its biochemical properties and catalytic performance. Results showed that the enzyme was identified as lipase by SDS-PAGE. The optimum temperature of lipase was 60℃ and stabilized within 30-60 ℃, while its optimum pH 9.0, and its activity was stable in pH range 6-9. Mg2＋ has a strong role in promoting its activity, while Zn2＋ and Na＋ have little effect on lipase, but Caz＋ and EDTA significantly inhibit its activity. After degradation for 30 d, the number-average molecular weight and weight- average molecular weight of PBS decreased, the crystalline particles decreased and the thermal stability also declined, which indicated that the strain has significant effect on PBS degradation.

关 键 词：聚丁二酸丁二醇酯 脂肪酶 分离纯化 生物降解 

分 类 号：X172[环境科学与工程—环境科学]