Molecular cloning and expression analysis of FTZ-F1 in the GIFT tilapia,Oreochromis niloticus  

作　　者：Jinling CAO Jianjie CHEN Zhongliang JIANG Yongju LUO Xi GAN 

机构地区：[1]State Key Laboratory of Ecological Animal Husbandry and Environmental Veternary Medicine,Shanxi Agricultural University,030801 Taigu,Shanxi,China [2]Guangxi University,530021 Nanning,China [3]Guangxi Fisheries Research Institute,530021 Nanning,China

出　　处：《Zoological Research》2012年第4期I0012-I0018,共7页动物学研究（英文）

基　　金：the Natural Science Foundation of Shanxi Province,China (2009021038);the Doctor Initial Foundation of Shanxi Agricultural University (XB2009003);the Science and Technology Innovational Foundation of Shanxi Agricultural University (2009005);the Nonprofit Research Institutes,Special Foundation for Operating Expenses of Basic Research Projects of Guangxi Zhuang Autonomous Region(2060302 GXIF-2008-01);the Scientific Research and Technological Development Projects of Nanning City(200801016B)

摘　　要：The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia(gfFTZ-F1).The full-length cDNA was isolated from the ovary,which included an open reading frame encoding a predicted protein of 486 amino acids.Sequence,tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts,though further study is needed to make any definitive conclusions.The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination. We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia （gfFTZ-F1）. The full-length cDNA was isolated from the ovary, which included an open reading frame encoding a predicted protein of 486 amino acids. Sequence, tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues. Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts, though further study is needed to make any definitive conclusions.

关 键 词：GIFT tilapia FTZ-F 1 CLONING EXPRESSION 

分 类 号：Q785[生物学—分子生物学] S965.125[农业科学—水产养殖]