棉籽粕固态发酵过程及其动力学模型构建  

Process of solid-state fermentation of cottonseed meal and its kinetic model

作　　者：吴逸飞[1] 孙宏[1] 姚晓红[1] 王新[1] 汤江武[1] 

机构地区：[1]浙江省农业科学院植物保护与微生物研究所,杭州310021

出　　处：《农业工程学报》2012年第13期199-204,共6页Transactions of the Chinese Society of Agricultural Engineering

基　　金：浙江省重大科技专项资助项目(2011C12010);杭州市重大科技创新专项资助项目(20092112A41)

摘　　要：为了研究枯草芽孢杆菌Bs-1在工业化条件下发酵棉籽粕的过程,该文采用槽式发酵方式,测定了发酵过程中物料堆温、含水率、粗蛋白、酸溶性蛋白和游离棉酚质量分数;并采用Logistic方程建立芽孢杆菌Bs-1生长模型,在其与粗蛋白、酸溶性蛋白和游离棉酚质量分数相关性分析的基础上,构建了固态发酵动力学模型并进行了发酵成本分析。结果表明:棉籽粕槽式发酵过程中的温度和含水率均呈现一定的变化规律;粗蛋白质量分数、酸溶性蛋白质量分数分别较发酵前提高了9.28%和46.51%,游离棉酚质量分数降低了42.31%。Bs-1的生长与粗蛋白质量分数(R2=0.831)和酸溶性蛋白质量分数(R2=0.867)呈正相关,与游离棉酚质量分数(R2=0.976)呈负相关;建立了棉籽粕固态发酵动力学模型。分析表明,该模型计算值与试验值能较好吻合,且发酵成本低。表明该模型可应用于工业化生产,对实现发酵棉籽粕的产业化生产,具有较好的参考价值。In order to investigate the changes of the concentrations of free gossypol（FG）,crude protein（CP） and acid soluble protein（ASP） in the solid-state fermentation of cottonseed meal by Bacillus subtilis Bs-1,the temperatures,moisture contents,as well as the concentrations of CP,ASP and FG were measured in windrow-type aerobic fermentation.A growth model of Bs-1 was established using the equation of logistic.The kinetic models of cottonseed meal fermentation were built by accessing the correlation between the growth of B.subtilis Bs-1 and the contents of CP,ASP,and FG.Results showed that both temperature and moisture had regular changing trends in the process of fermentation.The concentrations of CP and ASP increased by 9.28% and 46.51%,respectively,whereas the FG content dropped by 42.31% after fermentation.Correlations between Bs-1 number and CP（R2=0.831）/ASP（R2=0.867） were positive.The Bs-1 population was negatively correlated with FG content（R2=0.976）.The predicted values of the models agreed well with the experimental values in the verification experiment.The models can be used to predict the fermentation coursed by Bacillus subtilis Bs-1 under industrial-scale solid-state fermentation.

关 键 词：发酵 动力学 模型 粗蛋白 酸溶性蛋白 游离棉酚 棉籽粕 

分 类 号：S816.6[农业科学—饲料科学]