ATP合成关键酶基因APT1在雅致放射毛霉中的克隆表达  

Cloning and Experssion of Key Enzyme Gene APT1 for ATP Synthesis in Actinomucor elegans

作　　者：朱家荣[1] 杨善岩[1] 陈丽芬[1] 杨光辉[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310014

出　　处：《食品与发酵工业》2012年第6期43-47,共5页Food and Fermentation Industries

摘　　要：为构建遗传稳定的ATP高产工程菌,利用PCR技术扩增酿酒酵母ATP合成关键酶基因APT1,并将其克隆至质粒pCB1004-Pgpd的相应位点,得到有强启动子Pgpd驱动的APT1基因超表达质粒pCB1004-Pgpd-APT1。在PEG-CaCl2介导下,超表达质粒转化雅致放射毛霉原生质体,获得ATP高产工程菌。其ATP产量及摩尔转化率比出发菌株提高44.04%。To construct the engineering strain with genetic stability which can efficient to synthesize ATP,the key enzyme gene APT1 for ATP synthesis which from Saccharomyces cerevisiae was cloned by PCR.The sequence was cloned to corresponding site of pCB1004-Pgpd.Then the super expression plasmid pCB1004-Pgpd-APT1 which controlled by strong promoter Pgpd was obtained.PEG and CaCl2 mediated protoplast transformation of Actinomucor elegans with super expression plasmid was performed and ATP-overproducing transformants were obtained.The ATP yield and Moore conversion were increased 44.04% in comparison with those from original strain.

关 键 词：APT1 雅致放射毛霉 酿酒酵母 

分 类 号：Q78[生物学—分子生物学]