抑癌基因PTEN在甲状腺乳头状癌中的表达及临床意义  

The Expression and Clinical Significance of Tumor-suppressor Genes PTEN in Thyroid Papillary Carcinoma

作　　者：鲁金乐[1] 赵雪松[1] 何国庆[1] 聂春磊[1] 孙冰[1] 于家伟[1] 

机构地区：[1]哈尔滨医科大学第三附属医院,黑龙江哈尔滨150040

出　　处：《现代生物医学进展》2012年第21期4075-4078,共4页Progress in Modern Biomedicine

基　　金：黑龙江省自然科学基金(D200833)

摘　　要：目的:通过免疫组织化学方法检测PTEN基因在正常甲状腺组织、甲状腺良性肿瘤组织、甲状腺乳头状癌癌组织中的表达水平并进行比较,探讨其对甲状腺乳头状癌诊断和治疗的意义。方法:采用SP免疫组化方法,用已知阳性组织做阳性对照,以磷酸盐缓冲液(PBS)代替一抗做阴性对照,分别作HE染色和免疫组织化学染色。结果:PTEN蛋白在三组组织中的表达差异具有显著性(P<0.001);正常甲状腺组织、甲状腺良性肿瘤组织中的阳性率分别为100%和82.5%,均显著高于甲状腺癌组织中的45%(P<0.05),即PTEN在甲状腺癌中表达显著降低;PTEN在甲状腺乳头状癌淋巴结转移组和无淋巴结转移组阳性表达率分别为15%和60%,差异有显著性(x2=10.91,P=0.001);PTEN在甲状腺乳头状癌在包膜侵犯组和无侵犯组的阳性表达率分别为25.93%和60.61%,差异有显著性(x2=7.22,P=0.007);PTEN在甲状腺癌淋巴结转移组和包膜侵犯组的阳性表达强度显著低于无淋巴结转移和包膜侵犯组(P<0.01),有统计学意义。结论:PTEN基因表达的降低在甲状腺癌的发生和转移过程中起重要作用。Objective： To detect and analyse the expression of PTEN genes in normal thyroid, thyroid benign tumor tissue, and thyroid papillary carcinoma cancer tissue by immunohistochemical method. Meanwhile to investigate the thyroid papillary carcinoma diagnosis and treatment of significance. Methods： Adapt SP immunohistochemical method, took known positive tissue as positive control, took phosphate buffer （PBS） instead of antibodies as a negative control, all tissue were studied with HE dyeing and immunohistochemical staining. Results： In three groups, PTEN protein expressed in the organization with significant difference （P〈0.001）; the positive expression in normal thyroid and thyroid benign tumor tissue were 100% and 82.5% respectively which were significantly higher than the 45% of thyroid cancer group （P〈0.05）, namely the expression in the PTEN thyroid cancer significantly reduced; the expression rate of PTEN in thyroid papillary carcinoma lymph node metastasis group and without lymph node metastasis group were 15% and 60%, signifi- cantly （the chi-square = 10.91, P = 0.001）. PTEN in thyroid papillary carcinoma in coating infiinged group and no violation of the group positive expression rate were 25.93% and 60.61% and the difference was significantly （the chi-square = 7.22, P = 0.007）. The strength of positive PTEN in thyroid cancer lymph node metastasis group and the infringement of coated positive were significantly lower than lymph node metastasis and coated infringed group （P〈0.01） which reached a statistical significance. Conclusion： The reducing of the expression ofPTEN gene palys a key role in thyroid cancer and the process of disease transfer.

关 键 词：甲状腺乳头状癌 PTEN基因 免疫组织化学技术 淋巴结转移 

分 类 号：R736.1[医药卫生—肿瘤]