干酪乳杆菌对数生长期与稳定期蛋白质组比较分析  

Comparative Proteomic Analysis of Lactobacillus casei XM2-1 between Exponential and Stationary Phases

作　　者：乌日娜[1] 岳喜庆[1] 张和平[2] 

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110866 [2]内蒙古农业大学乳品生物技术与工程教育部重点实验室,内蒙古呼和浩特010018

出　　处：《食品科学》2012年第13期148-151,共4页Food Science

基　　金：国家自然科学基金项目(31025019;31000805);国家现代农业产业技术体系建设项目(nycytx-0501);国家"973"计划项目(2010CB134502);"十一五"国家科技支撑计划重点项目(2009BADC1B01);教育部创新团队发展计划项目(IRT0967)

摘　　要：研究建立干酪乳杆菌Lactobacillus casei XM2-1的蛋白质组双向电泳表达图谱,比较其在对数生长中期与稳定期初期的图谱差异。结果表明:有16个蛋白质点表达发生明显变化,经过基质辅助激光解吸/电离飞行时间质谱鉴定,其中有7个蛋白质点鉴定为应激蛋白质,分别为GroEL、DnaK、Hsp 20-1、Hsp 20-2和UspA蛋白质。可见,应激蛋白质可能在L.casei XM2-1稳定期对外界环境的抵御能力增强起到重要作用。Proteomic analysis was carried out to identify and characterize protein expression in Lactobacillus casei XM2-1. The cytosolic proteins of L. casei XM2-1 at the mid-exponential phase and early stationary phase were resolved by two- dimensional gel electrophoresis. Sixteen proteins revealed significant differences between two growth phases, and seven proteins were identified as stress proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI- TOF/MS）, which included GroEL, DnaK, Hsp 20-1, Hsp 20-2 and UspA. This will be helpful for improving our understanding of the lg-ATR response in L. casei.

关 键 词：干酪乳杆菌 蛋白质组 应激蛋白质 

分 类 号：TS201.3[轻工技术与工程—食品科学]