产抗氧化胞外多糖海洋细菌Bacillus subtilis OST23a的诱变育种  

Mutation of Marine Strain Bacillus subtilis OST23a to Obtain a High-Yield Antioxidant Exopolysaccharides-Producing Strain

作　　者：房耀维[1] 刘姝[1] 吕明生[1] 焦豫良[1] 陈丽[1] 王淑军[1] 

机构地区：[1]淮海工学院海洋学院,江苏省海洋资源开发研究院,江苏连云港222005

出　　处：《食品科学》2012年第13期187-190,共4页Food Science

基　　金：江苏省高校自然科学基础研究面上项目(08KJB550001)

摘　　要：利用产生抗氧化活性胞外多糖的海洋细菌Bacillus subtilis OST23a为出发菌株进行高产菌株的诱变选育。采用紫外、硫酸二乙酯复合诱变处理后筛选得到一株高产胞外多糖的优良突变菌株UD292,发酵产胞外多糖水平达到43.92mg/L,较出发菌株提高了6.26倍,且多糖的抗氧化活性没有显著变化。对突变菌株进行连续传代实验结果表明突变菌株遗传性状稳定。Bacillus subtilis OST23a, a marine bacterium, was mutagenized with both UV light and DES treatment to obtain a high-yield antioxidant exopolysaccharide （EPS）-producing strain named as UD292. The EPS yield of the mutant strain was 43.92 mg/L, which increased 6.26-fold as compared with the original strain. The EPS obtained from both strains showed no obvious difference in their antioxidant activity. The mutant strain remained stable in successive passages.

关 键 词：抗氧化胞外多糖 海洋细菌 诱变育种 

分 类 号：TS202.21[轻工技术与工程—食品科学]